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Imaging centrosomes and cilia in the mouse kidney
Masato Hoshi1, Jinzhi Wang1, Sanjay Jain2
1Division of Nephrology, Department of Medicine, Washington University School of Medicine, St Louis, MO, USA.
Methods in Cell Biology
|April 4, 2015
Summary
This study details methods for visualizing centrosomes and cilia in mouse kidneys. These techniques are crucial for understanding kidney development and diseases like cystic kidney disease.
Area of Science:
- Cell Biology
- Developmental Biology
- Renal Physiology
Background:
- Centrosomes and cilia are vital microtubule-based organelles involved in cellular signaling.
- Defects in the centrosome-cilium complex cause ciliopathies, impacting kidney development and homeostasis.
- Ciliary dysfunction is linked to cystic kidney disease pathogenesis.
Purpose of the Study:
- To provide detailed protocols for studying centrosomes and cilia in mammalian kidneys.
- To enable researchers to visualize, analyze, and test the function of these organelles in renal tissues.
- To support studies on kidney development and the molecular mechanisms of kidney diseases.
Main Methods:
- Isolation of kidneys from adult and embryonic mice.
- Protocols for labeling centrosomes and cilia within renal tissues.
- Ex vivo culture and fluorescence microscopy techniques for imaging.
Main Results:
- Established robust methods for preparing and imaging mouse kidney tissues.
- Demonstrated successful labeling and visualization of centrosomes and cilia.
- Provided a framework for functional and compositional analysis of these organelles in situ.
Conclusions:
- The presented techniques are essential for advancing research on kidney development and disease.
- Accurate visualization and analysis of centrosomes and cilia are key to understanding renal function.
- These methods will facilitate the study of ciliopathies and cystic kidney disease.
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