Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Focusing of Light in the Eye01:16

Focusing of Light in the Eye

7.8K
Light rays enter the eye through the cornea, a transparent dome-shaped tissue that is the eye's outermost layer. The cornea bends or refracts, light rays traveling to the pupil. The shape of the cornea determines how much of the light is bent and whether the image will be focused correctly on the retina at the back of the eye. Once the light has passed through both refraction layers, it converges into a single focal point onto a small area. This is where photoreceptors start transforming...
7.8K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.9K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.9K
Common Leveling Mistakes and Errors01:17

Common Leveling Mistakes and Errors

620
A survey team is tasked with determining the elevation difference between points Point A and Point B, separated by uneven terrain. They use a leveling instrument and a leveling rod.Common MistakesMisreading the Rod: During a backsight reading at Point A, the instrumentman observes the rod partially obscured by tall grass. Instead of reading 1.135 m, they mistakenly record 1.735 m due to the misalignment of the crosshair with the wrong graduation. This error adds 0.600 m to all subsequent...
620
Distance Corrections01:15

Distance Corrections

395
To achieve precise distance measurements, especially in surveying and construction, certain corrections must be applied to account for potential sources of error like the standardization errors, temperature variations, and slope adjustments.Standardization error emerges when measurement equipment undergoes changes, such as wear, repairs, or weather impacts. To address this, surveyors compare the equipment’s readings to a standard. This process identifies any deviation that might lead to...
395
Difference from Background: Limit of Detection01:05

Difference from Background: Limit of Detection

10.1K
The limit of detection (LOD) is the smallest amount of analyte that can be distinguished from the background noise. The LOD value corresponds to the concentration at which the analyte signal is three times larger than the standard deviation of the blank signal. Below this value, the analyte signal cannot be differentiated from the background noise. It is calculated by dividing the calibration slope by 3 times the standard deviation of the blank signals.
The LOD indicates the presence or absence...
10.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Response to teprotumumab in thyroid eye disease patients of long duration and high clinical activity score.

Orbit (Amsterdam, Netherlands)·2026
Same author

Histiocytic Sarcoma of the Eyelid: An Uncommon Presentation of a Rare Neoplasm.

Ophthalmic plastic and reconstructive surgery·2026
Same author

Severe Congenital Ptosis Repair: A Report by the American Academy of Ophthalmology.

Ophthalmology·2026
Same author

The Role of Corticosteroids in the Management of Orbital Cellulitis: A Report by the American Academy of Ophthalmology.

Ophthalmology·2026
Same author

Spontaneous regression of a presumed orbitocranial eosinophilic granuloma in an adult patient: illustrative case.

Journal of neurosurgery. Case lessons·2026
Same author

A rare case of infantile dacryoadenitis: a case report.

Journal of AAPOS : the official publication of the American Association for Pediatric Ophthalmology and Strabismus·2026
Same journal

Hyper Immunoglobulin E Syndrome With Conjunctival Molluscum Contagiosum.

Ophthalmic plastic and reconstructive surgery·2026
Same journal

Ethmoiditis Mimicking Acute Dacryocystitis-An Underreported Pathology.

Ophthalmic plastic and reconstructive surgery·2026
Same journal

Orbital Inflammatory Syndrome Associated with Bispecific T-Cell Engager Therapy: Case and Literature Review.

Ophthalmic plastic and reconstructive surgery·2026
Same journal

A Rare Pedunculated Lobular Capillary Hemangioma of the Conjunctiva.

Ophthalmic plastic and reconstructive surgery·2026
Same journal

Core Oculoplastic Procedure Skills for the Comprehensive Ophthalmologist: A Consensus by Delphi Methodology.

Ophthalmic plastic and reconstructive surgery·2026
Same journal

The Role of Corneal Sensation in Facial Nerve Paralysis.

Ophthalmic plastic and reconstructive surgery·2026
See all related articles

Related Experiment Video

Updated: Apr 15, 2026

Video Movement Analysis Using Smartphones ViMAS: A Pilot Study
07:51

Video Movement Analysis Using Smartphones ViMAS: A Pilot Study

Published on: March 14, 2017

17.4K

Margin Reflex Distance: Differences Based on Camera and Flash Positions.

Catherine J Choi1, Jonathan C Chou, Daniel R Lefebvre

  • 1*Ophthalmic Plastic Surgery, Massachusetts Eye and Ear Infirmary; and †Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts, U.S.A.

Ophthalmic Plastic and Reconstructive Surgery
|April 7, 2015
PubMed
Summary
This summary is machine-generated.

Camera flash position significantly impacts margin reflex distance (MRD) measurements. Cameras with near-coaxial light sources provide the most accurate photographic MRD1, aligning closely with clinical findings.

More Related Videos

Author Spotlight: An Accurate and Quantitative Approach to Study Visual Feature Selectivity of the Optokinetic Reflex in Mice
09:28

Author Spotlight: An Accurate and Quantitative Approach to Study Visual Feature Selectivity of the Optokinetic Reflex in Mice

Published on: June 23, 2023

4.1K
Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.5K

Related Experiment Videos

Last Updated: Apr 15, 2026

Video Movement Analysis Using Smartphones ViMAS: A Pilot Study
07:51

Video Movement Analysis Using Smartphones ViMAS: A Pilot Study

Published on: March 14, 2017

17.4K
Author Spotlight: An Accurate and Quantitative Approach to Study Visual Feature Selectivity of the Optokinetic Reflex in Mice
09:28

Author Spotlight: An Accurate and Quantitative Approach to Study Visual Feature Selectivity of the Optokinetic Reflex in Mice

Published on: June 23, 2023

4.1K
Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.5K

Area of Science:

  • Ophthalmology
  • Medical Imaging
  • Biomedical Engineering

Background:

  • Photographic margin reflex distance (MRD) is a valuable clinical measurement.
  • Accuracy of photographic MRD can be influenced by imaging parameters, including flash position.
  • Understanding these influences is crucial for reliable ophthalmic assessments.

Purpose of the Study:

  • To investigate the impact of camera flash position on margin reflex distance (MRD) measurements.
  • To compare photographic MRD measurements across different camera types and flash configurations.

Main Methods:

  • Prospective enrollment of subjects without ophthalmic disease.
  • Clinical measurement of MRD1 and interpalpebral fissure.
  • Digital photography using various cameras (dSLR-pop, dSLR-ring, point-and-shoot, smartphone) with flash in four positions (0°, 90°, 180°, 270°).
  • Image analysis using ImageJ software for MRD1, interpalpebral fissure, white-to-white, and nasal limbus to light reflex measurements.

Main Results:

  • No significant differences between clinical and photographic MRD1 using dSLR-ring, point-and-shoot, and smartphone cameras.
  • Statistically significant differences in MRD1 measurements with the dSLR-pop camera, particularly in the upright position and between upright and inverted orientations.
  • No significant differences observed for interpalpebral fissure, white-to-white, or nasal limbus to light reflex measurements across all cameras and positions.

Conclusions:

  • Cameras employing a near-coaxial light source and aperture yield photographic MRD1 values most consistent with clinical measurements.
  • Flash position and camera type critically affect the accuracy of photographic MRD1.
  • Near-coaxial lighting is recommended for reliable photographic MRD assessments.