Nitric oxide-mediated pain processing in the spinal cord
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Updated: Apr 15, 2026

Preparation of Acute Spinal Cord Slices for Whole-cell Patch-clamp Recording in Substantia Gelatinosa Neurons
Published on: January 18, 2019
1Institut für Pharmakologie und Toxikologie, Universität Witten/Herdecke, ZBAF, Stockumer Str. 10, 58453, Witten, Germany, Achim.Schmidtko@uni-wh.de.
Abstract:
A large body of evidence indicates that nitric oxide (NO) plays an important role in the processing of persistent inflammatory and neuropathic pain in the spinal cord. Several animal studies revealed that inhibition or knockout of NO synthesis ameliorates persistent pain. However, spinal delivery of NO donors caused dual pronociceptive and antinociceptive effects, pointing to multiple downstream signaling mechanisms of NO. This review summarizes the localization and function of NO-dependent signaling mechanisms in the spinal cord, taking account of the recent progress made in this field.
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