Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Serum Biomarkers in Paediatric Neurosurgery: Traumatic Brain Injury and Beyond.

Advances and technical standards in neurosurgery·2026

Same author

Movement Disorders in Children: Treatment Algorithm and Surgical Options.

Advances and technical standards in neurosurgery·2026

Same author

<i>Asplenium yishuiensis</i> (Aspleniaceae), a New Wintergreen and Medicinal Fern from Northern China, Achieves Freezing Tolerance via a Calcium-Mediated Osmotic Adjustment Pathway.

Plants (Basel, Switzerland)·2026

Same author

Research on the Physiological Response Mechanism and Expression of Key Leaf Color Genes in 'Duojiao' Crabapple Under Partial Shading.

Plants (Basel, Switzerland)·2026

Same author

A Crisscross-Enhanced Groupers and Moray Eels Optimization Algorithm: Benchmark Test and Production Optimization.

Biomimetics (Basel, Switzerland)·2026

Same author

Isoform-specific cofactor recruitment through the intrinsically disordered N-terminus of p63 underlies differential transcriptional activities.

bioRxiv : the preprint server for biology·2026

Same journal

Tunable self-assembling cellular microarray for single-neutrophil vital and suicidal extracellular traps.

Lab on a chip·2026

Same journal

Precise programmable tumor cell subpopulation sorting <i>via</i> an electromagnetic microfluidic platform.

Lab on a chip·2026

Same journal

Bridging dimensions: combining one- and two-photon 3D printing for microfluidic device fabrication.

Lab on a chip·2026

Same journal

Microfluidic rare cell analysis beyond counting: workflow design from enrichment to multi-omics.

Lab on a chip·2026

Same journal

A sperm racetrack to separate sperm by swim speed.

Lab on a chip·2026

Same journal

Controlled encapsulation and droplet size prediction in two-step microfluidic double emulsions.

Lab on a chip·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Apr 15, 2026

Generation of a Human iPSC-Based Blood-Brain Barrier Chip

Generation of a Human iPSC-Based Blood-Brain Barrier Chip

Published on: March 2, 2020

Microengineered peripheral nerve-on-a-chip for preclinical physiological testing.

Renee M Huval¹, Oliver H Miller, J Lowry Curley

¹Tulane University - Biomedical Engineering, New Orleans, Louisiana, USA. mooremj@tulane.edu.

|April 9, 2015

Summary

This summary is machine-generated.

This study introduces a novel 3D neural culture model using dual hydrogels to mimic nerve tissue in vitro. This advanced model enables physiologically relevant electrophysiological recordings, improving drug compound screening.

More Related Videos

Fabrication and Validation of an Organ-on-chip System with Integrated Electrodes to Directly Quantify Transendothelial Electrical Resistance

Fabrication and Validation of an Organ-on-chip System with Integrated Electrodes to Directly Quantify Transendothelial Electrical Resistance

Published on: September 26, 2017

Scalable Fabrication of Stretchable, Dual Channel, Microfluidic Organ Chips

Scalable Fabrication of Stretchable, Dual Channel, Microfluidic Organ Chips

Published on: October 20, 2018

Related Experiment Videos

Last Updated: Apr 15, 2026

Generation of a Human iPSC-Based Blood-Brain Barrier Chip

Generation of a Human iPSC-Based Blood-Brain Barrier Chip

Published on: March 2, 2020

Fabrication and Validation of an Organ-on-chip System with Integrated Electrodes to Directly Quantify Transendothelial Electrical Resistance

Fabrication and Validation of an Organ-on-chip System with Integrated Electrodes to Directly Quantify Transendothelial Electrical Resistance

Published on: September 26, 2017

Scalable Fabrication of Stretchable, Dual Channel, Microfluidic Organ Chips

Scalable Fabrication of Stretchable, Dual Channel, Microfluidic Organ Chips

Published on: October 20, 2018

Area of Science:

Neuroscience
Biomedical Engineering
Drug Discovery

Background:

High attrition rates of pharmaceutical compounds necessitate improved in vitro testing.
Mimicking neurological architecture and physiology in vitro remains challenging.
Organotypic nerve tissue models are needed for predictive drug screening.

Purpose of the Study:

To develop a microscale, organotypic model of nerve tissue for predictive in vitro testing.
To create a biomimetic sensory neural fiber tract for electrophysiological recordings.
To enable physiologically relevant readouts for drug compound evaluation.

Main Methods:

Utilized a dual hydrogel construct with explants from rat embryonic dorsal root ganglia.
Developed a microengineered, spatially-controlled sensory neural fiber tract.
Performed intra- and extra-cellular electrophysiological recordings.

Main Results:

The 3D neural cultures structurally and functionally mimicked in vivo afferent sensory peripheral fibers.
The dual hydrogel system facilitated high-density, parallel, fasciculated neural growth.
The model produced outputs resembling clinical compound action potential (CAP) and nerve fiber density (NFD) tests.

Conclusions:

This advanced in vitro model offers a cost-effective approach for selecting promising pharmaceutical compounds.
The biomimetic neural growth assay supports mechanistic studies with physiologically relevant readouts.
The flexible 3D hydrogel constructs allow for systematic culture, perturbation, and testing.