Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Voltammetric Techniques: Pulse Voltammetry01:17

Voltammetric Techniques: Pulse Voltammetry

2.2K
Differential-pulse voltammetry (DPV) is a type of voltammetry that involves applying a series of voltage pulses to an electrochemical cell while measuring the resulting current. In DPV, the differential pulse or small potential pulses are superimposed on a linear potential sweep. The magnitude of these pulses is typically small, often in the millivolt range. Each voltage pulse lasts a short duration, usually in the order of a few milliseconds, and is applied at regular intervals along the...
2.2K
Voltammetric Techniques: Linear-Scan (E vs Time)01:12

Voltammetric Techniques: Linear-Scan (E vs Time)

1.9K
Polarography is a classical voltammetric technique used to analyze electrochemical reactions. This method applies a linear potential sweep to a dropping mercury electrode (DME), and the resulting current is measured. A dropping mercury electrode is commonly used as the working electrode in polarography. It consists of a capillary tube filled with mercury, where the tiny droplet forms at the tip. This droplet continuously drops from the capillary, creating a new electrode surface for each...
1.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Total Synthesis of Conjugation-Ready Sulfated Red Algae Carrageenan Oligosaccharides for Sensing Applications.

Journal of the American Chemical Society·2026
Same author

Targeting primary and metastatic ovarian cancer with a peptide derived from the human NAF-1/CISD2 protein.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026
Same author

Methods for studying the effects of phosphorylation patterns in proteins.

Biochemical Society transactions·2026
Same author

Expeditious Synthesis of Multiglycosylated Peptides for Sensing of <i>Listeria monocytogenes</i>.

Journal of medicinal chemistry·2025
Same author

Monosaccharide-Derived Enantioselectivity in SWCNT Chemoresistive VOC Sensing.

Chemistry (Weinheim an der Bergstrasse, Germany)·2025
Same author

FibrilPaint to determine the length of Tau amyloids in fluids.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same journal

Untreated Rosehip Powder/Poly(Lactic Acid)/Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate) Electrospun Mats for Wound Dressing Applications.

Biopolymers·2026
Same journal

Synthesis, Characterization, and Antidiabetic Evaluation of Sequence-Modified Liraglutide Analogs in a Drosophila melanogaster Model.

Biopolymers·2026
Same journal

Fabrication of an Antibacterial Alginate/Chitosan Hydrogel Dressing Loaded With CuO Nanoparticles for Wound Dressing Applications.

Biopolymers·2026
Same journal

Effect of Chitosan-Alginate Polyelectrolyte Complex Formation and Multilayer Polymer Configuration on the Characteristics of 3D-Printed Metronidazole-Loaded Periodontal Films.

Biopolymers·2026
Same journal

Phenolic Grafting of Oxidized Cellulose Nanofibers Using Ferulic Acid: Structural and Antioxidant Analysis Toward Bioactive Nanomaterials.

Biopolymers·2026
Same journal

Detection of a Target Nucleic Acid by Ligation-Assisted Fluorescence Enhancement of a Peptide Nucleic Acid (PNA) Twin Probe via Disulfide Binding.

Biopolymers·2026
See all related articles

Related Experiment Video

Updated: Apr 15, 2026

Assaying Protein Kinase Activity with Radiolabeled ATP
08:05

Assaying Protein Kinase Activity with Radiolabeled ATP

Published on: May 26, 2017

19.6K

A highly sensitive square wave voltammetry based biosensor for kinase activity measurements.

Elza Snir1, Einav Amit1, Assaf Friedler1

  • 1Institute of Chemistry and the Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel.

Biopolymers
|April 9, 2015
PubMed
Summary
This summary is machine-generated.

A novel electrochemical biosensor enables ultrasensitive, label-free detection of protein kinase activity by monitoring peptide monolayer changes. This method offers significantly improved sensitivity and dynamic range compared to previous techniques.

Keywords:
electrochemistrykinase biosensorpeptide monolayer

More Related Videos

Use of Enzymatic Biosensors to Quantify Endogenous ATP or H2O2 in the Kidney
10:00

Use of Enzymatic Biosensors to Quantify Endogenous ATP or H2O2 in the Kidney

Published on: October 12, 2015

12.4K
Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors
10:59

Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors

Published on: February 10, 2014

10.7K

Related Experiment Videos

Last Updated: Apr 15, 2026

Assaying Protein Kinase Activity with Radiolabeled ATP
08:05

Assaying Protein Kinase Activity with Radiolabeled ATP

Published on: May 26, 2017

19.6K
Use of Enzymatic Biosensors to Quantify Endogenous ATP or H2O2 in the Kidney
10:00

Use of Enzymatic Biosensors to Quantify Endogenous ATP or H2O2 in the Kidney

Published on: October 12, 2015

12.4K
Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors
10:59

Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors

Published on: February 10, 2014

10.7K

Area of Science:

  • Electrochemistry
  • Biosensors
  • Biochemistry

Background:

  • Protein kinases play crucial roles in cellular signaling pathways.
  • Dysregulation of protein kinase activity is implicated in various diseases, including cancer.
  • Accurate measurement of kinase activity is essential for research and drug development.

Purpose of the Study:

  • To develop an ultrasensitive, label-free electrochemical biosensor for determining protein kinase activity.
  • To investigate the use of a peptide monolayer on a gold electrode for kinase activity detection.
  • To compare the performance of the developed sensor with previously reported methods.

Main Methods:

  • Fabrication of a gold electrode modified with a unique peptide monolayer.
  • Utilizing square wave voltammetry (SWV) to measure electrochemical signal changes.
  • Monitoring the disorder induced in the peptide monolayer upon phosphorylation by kinases.

Main Results:

  • The biosensor demonstrated ultrasensitive, label-free determination of protein kinase activity.
  • Phosphorylation induced disorder in the peptide monolayer, leading to increased electrochemical signal.
  • Achieved a sensitivity of 100 nM and a dynamic range of 100 nM to 11 μM.
  • Exhibited an order of magnitude higher sensitivity and two orders of magnitude wider dynamic range than a previous impedimetric method.

Conclusions:

  • The developed electrochemical biosensor provides a highly sensitive and label-free platform for kinase activity assessment.
  • The sensor's mechanism relies on detecting structural changes in a peptide monolayer.
  • This approach offers significant advantages over existing methods for kinase activity measurement.