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Updated: Apr 15, 2026
Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
Published on: March 24, 2017
[Prediction of epitopes of Rv1410c Mycobacterium tuberculosis protein using DNAStar software]
Jiangying Li1, Xuejuan Bai2, Yan Liang2
1Hebei North University, Zhangjiakou 075000; PLA Key Laboratory of Tuberculosis Control and Prevention, Tuberculosis Research Institute of PLA, No. 309 Hospital of Chinese PLA, Beijing 100000, China.
Objective:
To predict the epitopes of Rv1410c Mycobacterium tuberculosis (MTB) protein.
Methods:
Rv1410c MTB amino acid sequence was saved and edited using Editseq software of DNAStar package, and then Protean software was used to analyze the amino acid sequence and predict the secondary structures, B cell epitopes and T cell epitopes of Rv1410c MTB. Finally, BLAST was utilized to assess its homology with human epitopes.
Results:
The Rv1410c MTB protein had abundant secondary structures. It contained a higher proportion of potential B cell epitopes located at 1-10, 67-77, 129-137, 189-205, 222-235, 253-267, 296-306, 330-338, 359-367, 462-467, 494-517 amino acid residues or nearby. Theses epitopes contained beta angle structure mostly, presenting with better antigenicity, higher surface probability and flexibility. There were also several potential T cell epitopes located at 16-37, 84-102, 108-115, 137-160, 202-207, 219-222, 245-263, 321-325, 355-362, 387-391, 417-445, 486-494 amino acid residues or nearby.
Conclusion:
Rv1410c MTB protein contains both potential B cell antigen epitopes and potential T cell antigen epitopes.
