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Round window membrane and perilymph in experimental otitis media with effusion
M Suzuki1, H Kawauchi, T Fujiyoshi
1Department of Otolaryngology, Medical College of Oita, Japan.
The Annals of Otology, Rhinology, and Laryngology
|December 1, 1989
Summary
Middle ear effusion (MEE) caused by immune complexes alters inner ear fluid composition. This study shows increased proteins and inflammatory mediators in perilymph (PL) during experimental otitis media with effusion (OME).
Area of Science:
- Otolaryngology
- Immunology
- Biochemistry
Background:
- Middle ear effusion (MEE) is a common condition, often associated with otitis media with effusion (OME).
- The biochemical changes in perilymph (PL) due to MEE are not fully understood.
Purpose of the Study:
- To investigate how MEE affects the biochemical composition of perilymph.
- To determine if immune complexes in the middle ear influence PL composition.
Main Methods:
- Experimental otitis media with effusion (OME) was induced in chinchillas using immune complexes.
- Perilymph was collected at different time points (4, 10, 21 days) post-induction.
- Concentrations of albumin, immunoglobulin G, histamine, and prostaglandin E2 (PGE2) in PL were measured.
Main Results:
- Significantly elevated levels of albumin, IgG, histamine, and PGE2 were found in the perilymph of ears with induced OME compared to controls.
- Increased passage of 3H-PGE2 into perilymph was observed in affected ears.
Conclusions:
- Immune complexes in the middle ear significantly alter the biochemical environment of the inner ear's perilymph.
- Middle ear effusion, induced by immune complexes, impacts the perilymph's biochemical milieu.