Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Homomultimeric FAP Inhibitor-Based Radioligands for Cancer Theranostics: Design Principles, Structure-Function Relationships, and Preclinical Performance.

Molecules (Basel, Switzerland)·2026

Same author

Long-term outcomes in medullary thyroid cancer patients treated with [<sup>177</sup>Lu]Lu-DOTAGA.FAPi dimer therapy.

EJNMMI research·2026

Same author

Measurement of Line Width and Anisotropy in <i>C</i><sub>3</sub>/<i>C</i><sub>4</sub>-Symmetric Gd(III) Complexes.

Inorganic chemistry·2026

Same author

Volume and Tempo: Cortical Excitability and Trial-to-Trial Consistency of Auditory Responses Distinguish Psychosis Biotypes.

Research square·2026

Same author

Protein binding regulates complex configuration: comparative analysis of three dynamically racemic europium(iii) complexes.

RSC advances·2026

Same author

Absolute Temperature Mapping Using Chiral Terbium Parashift Complexes for MRI Thermometry.

Chemical & biomedical imaging·2026

Same journal

Piperazine Derivatives Carrying an Imidazole Ring as Selective Modulators of Human Carbonic Anhydrase Isozymes.

ChemMedChem·2026

Same journal

Discovery of Ligands for the TNFR1 Extracellular Domain Using Fragment-Based Drug Discovery.

ChemMedChem·2026

Same journal

An Ugi-Joullié Platform for Medicinally Relevant 1,4-Dihydrobenzothiazines.

ChemMedChem·2026

Same journal

Metal-Drug Complexes as Long-Release Application for Antimalarial PfFNT-Inhibitors.

ChemMedChem·2026

Same journal

Speciation Studies of Monosubstituted Pt, Mo, and W Decavanadates in Culture Media and In Vitro Activity Against Trypanosoma cruzi.

ChemMedChem·2026

Same journal

Novel Xanthene Derivatives for Neuroprotection in Alzheimer's Disease-Synthesis and Biological Assessment.

ChemMedChem·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jan 17, 2026

Automated Preparation of [68Ga]Ga-3BP-3940 on a Synthesis Module for PET Imaging of the Tumor Microenvironment

Automated Preparation of [⁶⁸Ga]Ga-3BP-3940 on a Synthesis Module for PET Imaging of the Tumor Microenvironment

Published on: April 25, 2025

Approaching 'Kit-Type' Labelling with (68)Ga: The DATA Chelators.

Johanna Seemann¹, Bradley P Waldron^2,3, Frank Roesch²

¹Institute of Nuclear Chemistry, Johannes Gutenberg University, Fritz-Strassmann-Weg 2, 55128 Mainz (Germany). Johanna.Seemann@uni-mainz.de.

|April 23, 2015

Summary

This summary is machine-generated.

New DATA chelators offer faster, milder radiolabeling with Gallium-68 (68Ga) compared to traditional DOTA-based agents. This breakthrough shows great promise for Gallium-68 Positron Emission Tomography (PET) molecular imaging applications.

Keywords:

chelates gallium-68 ligand design macrocyclic ligands radiopharmaceuticals

More Related Videos

Radiosynthesis, Quality Control, and Small Animal Positron Emission Tomography Imaging of 68Ga-Labelled Nano Molecules

Radiosynthesis, Quality Control, and Small Animal Positron Emission Tomography Imaging of ⁶⁸Ga-Labelled Nano Molecules

Published on: October 4, 2024

Development of a 68Gallium-Labeled D-Peptide PET Tracer for Imaging Programmed Death-Ligand 1 Expression

Development of a 68Gallium-Labeled D-Peptide PET Tracer for Imaging Programmed Death-Ligand 1 Expression

Published on: February 3, 2023

Related Experiment Videos

Last Updated: Jan 17, 2026

Automated Preparation of [68Ga]Ga-3BP-3940 on a Synthesis Module for PET Imaging of the Tumor Microenvironment

Automated Preparation of [⁶⁸Ga]Ga-3BP-3940 on a Synthesis Module for PET Imaging of the Tumor Microenvironment

Published on: April 25, 2025

Radiosynthesis, Quality Control, and Small Animal Positron Emission Tomography Imaging of 68Ga-Labelled Nano Molecules

Radiosynthesis, Quality Control, and Small Animal Positron Emission Tomography Imaging of ⁶⁸Ga-Labelled Nano Molecules

Published on: October 4, 2024

Development of a 68Gallium-Labeled D-Peptide PET Tracer for Imaging Programmed Death-Ligand 1 Expression

Development of a 68Gallium-Labeled D-Peptide PET Tracer for Imaging Programmed Death-Ligand 1 Expression

Published on: February 3, 2023

Area of Science:

Radiochemistry
Nuclear Medicine
Molecular Imaging

Background:

Novel tri-anionic ligands, DATA chelators (6-amino-1,4-diazepine-triacetic acid derivatives), have been developed for Gallium-68 (68Ga) chelation.
Existing macrocyclic chelators, such as DOTA, DO3A, and DO2A, require specific conditions for efficient radiolabeling.

Purpose of the Study:

To systematically evaluate the Gallium-68 (68Ga) labeling efficiency of four novel DATA chelators: DATA(M), DATA(P), DATA(Ph), and DATA(PPh).
To compare the radiolabeling performance of DATA chelators with established macrocyclic chelators.

Main Methods:

Synthesis and characterization of four DATA chelator derivatives.
Gallium-68 (68Ga) radiolabeling experiments under varying conditions.
Analysis of labeling kinetics, yield, and purity.

Main Results:

DATA chelators demonstrated rapid and quantitative radiolabeling with Gallium-68 (68Ga).
Labeling occurred under significantly milder conditions compared to DOTA-based chelators.
All four evaluated DATA chelators showed excellent performance for 68Ga chelation.

Conclusions:

DATA chelators represent a promising new class of ligands for Gallium-68 (68Ga) radiolabeling.
Their superior labeling kinetics and milder conditions make them highly suitable for 68Ga Positron Emission Tomography (PET) applications.
These findings support the potential of DATA chelators in advancing molecular imaging.