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Updated: Apr 14, 2026
Delivery of the Cas9/sgRNA Ribonucleoprotein Complex in Immortalized and Primary Cells via Virus-like Particles "Nanoblades"
Published on: March 31, 2021
Direct Nuclear Delivery of DNA by Photothermal Nanoblade
Ting-Hsiang Wu1, Yi-Chien Wu2, Enrico Sagullo3
1Department of Mechanical and Aerospace Engineering, UCLA, Los Angeles, CA, USA Department of Pathology and Laboratory Medicine, UCLA, Los Angeles, CA, USA.
Abstract:
We demonstrate direct nuclear delivery of DNA into live mammalian cells using the photothermal nanoblade. Pulsed laser-triggered cavitation bubbles on a titanium-coated micropipette tip punctured both cellular plasma and nuclear membranes, which was followed by pressure-controlled delivery of DNA into the nucleus. High-level and efficient plasmid expression in different cell types with maintained cell viability was achieved.
