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Achieving room temperature DNA amplification by dialling in destabilization
B Safeenaz Alladin-Mustan1, Catherine J Mitran, Julianne M Gibbs-Davis
1Department of Chemistry, University of Alberta, Edmonton, Alberta T6E 2G2, Canada. gibbsdavis@ualberta.ca.
Abstract:
The ability to amplify nucleic acid biomarkers at room temperature has remained elusive despite the great need of diagnostics suitable for the point of care. To exponentially amplify DNA within a wide range of ambient temperatures (18-26 °C), we explore combining two destabilizing elements in our isothermal lesion-induced DNA amplification system. We demonstrate rapid DNA amplification at the bench without a heat source.
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