Microfluidic chip-capillary electrophoresis device for the determination of urinary metabolites and proteins

Microfluidic chip-CE (MC-CE) devices have caught recent attention for diagnostic applications in urine. This is due to the successes reported in handling real urine samples by integrating microfluidic chips (MC) with analyte enrichment and sample cleanup to CE with high separation efficiency and sensitive analyte detection. Here, we review the determination of urinary metabolites and proteins by MC-CE devices within the past 7 years. The application scope for MC-CE integrated devices was found to exceed the use of either technique alone, showing comparable performance to laser-induced fluorescence detection using less sensitive UV detectors, offering the flexibility to handle difficult urine samples with on-chip dilution and online standard addition and delivering enhanced performance as compared with commercial microfluidic chip electrophoresis chips.