Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A novel, wearable, in-ear EEG technology to assess sleep and daytime sleepiness.

Bioelectronic medicine·2026

Same author

Methods for Wearable-Derived Pulse Rate Measures and Their Application to Modeling the Relationship between Pulse Rate and Motor Activity in Narcolepsy Type 1.

Digital biomarkers·2026

Same author

Functional Regression Methods for Estimating 24-Hour Ambulatory Blood Pressure and Heart Rate Parameters in Narcolepsy Type 1.

Digital biomarkers·2026

Same author

The Value of Internal Positive Controls and Synthetic Calibrators for Performance Assessment of Breast Carcinoma Estrogen Receptor Immunohistochemistry Assay.

Laboratory investigation; a journal of technical methods and pathology·2026

Same author

Classification and clustering on nocturnal polysomnography: distinctions and overlaps between central disorders of hypersomnolence.

Sleep·2025

Same author

New standards in HER2-low testing: the CASI-01 comparative methods study.

EBioMedicine·2025

Same journal

Seeing, Interpreting, and Trusting Images in the Age of Artificial Intelligence.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026

Same journal

P63 and Tight Junctions Targeted for Therapy in Human Salivary Duct Adenocarcinoma Cells.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026

Same journal

Human iPSC-Derived Blood Vessel Organoids for Studying Chronic Hypoxia-Induced Microvascular Dysfunction.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026

Same journal

From Manual to Macro: A Reproducible Fiji Workflow for Semi-automated Collagen Fibril Diameter Quantification in Transmission Electron Microscopy.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026

Same journal

ADAMTS1 Is Required for Ventral Abdominal Wall Closure.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026

Same journal

FGF2 Boost for Driving Forebrain Organoid Maturation Under Static Conditions.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Apr 13, 2026

Synthetic Antigen Controls for Immunohistochemistry

Synthetic Antigen Controls for Immunohistochemistry

Published on: August 23, 2021

Standardizing Immunohistochemistry: A New Reference Control for Detecting Staining Problems.

Seshi R Sompuram¹, Kodela Vani¹, Brian Tracey²

¹Medical Discovery Partners LLC, Boston, Massachusetts (SRS,KV,SAB)

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society

|May 6, 2015

Summary

This summary is machine-generated.

A novel immunohistochemistry (IHC) control using peptide-coated beads offers standardized breast cancer testing. These "IHControls" ensure reliable detection of human epidermal growth factor receptor 2, estrogen receptor, and progesterone receptor, enhancing diagnostic accuracy.

Keywords:

IHControl bead immunohistochemistry peptide quality control validation

More Related Videos

Standardized Processing for Formalin-Fixed, Paraffin-Embedded Cell Pellet Immunohistochemistry Controls

Standardized Processing for Formalin-Fixed, Paraffin-Embedded Cell Pellet Immunohistochemistry Controls

Published on: July 27, 2022

Identification of Orexin and Endocannabinoid Receptors in Adult Zebrafish Using Immunoperoxidase and Immunofluorescence Methods

Identification of Orexin and Endocannabinoid Receptors in Adult Zebrafish Using Immunoperoxidase and Immunofluorescence Methods

Published on: June 25, 2019

Related Experiment Videos

Last Updated: Apr 13, 2026

Synthetic Antigen Controls for Immunohistochemistry

Synthetic Antigen Controls for Immunohistochemistry

Published on: August 23, 2021

Standardized Processing for Formalin-Fixed, Paraffin-Embedded Cell Pellet Immunohistochemistry Controls

Standardized Processing for Formalin-Fixed, Paraffin-Embedded Cell Pellet Immunohistochemistry Controls

Published on: July 27, 2022

Identification of Orexin and Endocannabinoid Receptors in Adult Zebrafish Using Immunoperoxidase and Immunofluorescence Methods

Identification of Orexin and Endocannabinoid Receptors in Adult Zebrafish Using Immunoperoxidase and Immunofluorescence Methods

Published on: June 25, 2019

Area of Science:

Biotechnology
Cancer Diagnostics
Immunohistochemistry

Background:

Standardized immunohistochemistry (IHC) controls are crucial for accurate breast cancer testing.
Current tissue-based controls can present challenges in reproducibility and standardization across laboratories.
Need for a robust control system to validate the analytical performance of IHC assays.

Purpose of the Study:

To introduce and evaluate a new standardized IHC control system, termed "IHControls", for breast cancer biomarker testing.
To assess the sensitivity and specificity of IHControls in detecting common issues in IHC staining procedures.
To compare the performance of IHControls against traditional tissue-based controls.

Main Methods:

Development of IHControls using formalin-fixed peptide antigens (HER2, ER, PR) covalently attached to glass beads.
Suspension of antigen-coated beads in a liquid matrix that solidifies on a microscope slide.
Testing IHControls by simulating various staining problems, including reagent degradation and suboptimal antigen retrieval conditions (time, temperature).

Main Results:

IHControls demonstrated sensitivity in detecting primary and secondary reagent degradation, comparable to tissue controls.
The controls effectively identified aberrations in antigen retrieval, mirroring the performance of tissue controls.
Specificity testing confirmed that each bead reacted only with its corresponding IHC antibody, validating assay specificity.

Conclusions:

IHControls are capable of verifying the analytical components of immunohistochemical staining, similar to tissue controls.
The bead-based format allows for bulk preparation, promoting day-to-day reproducibility and inter-laboratory standardization.
IHControls represent a promising advancement for standardized and reliable breast cancer biomarker assessment.