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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
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Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
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Positron Emission Tomography (PET) is a medical imaging technique that provides crucial insights into the body's physiological functions at a molecular level. It is an indispensable resource for diagnosing, staging, and monitoring various illnesses, notably cancer, neurological disorders, and cardiovascular conditions.
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DefinitionComputed Tomography (CT) of the genitourinary (GU) tract is a non-invasive imaging modality that utilizes X-rays and computer processing to generate detailed cross-sectional images of the urinary system, encompassing the kidneys, ureters, bladder, and adjacent structures such as the adrenal glands.PurposeCT scans of the GU tract serve several diagnostic and therapeutic purposes, including:Diagnosis of Urinary Tract Diseases: Detects kidney stones, tumors, cysts, and congenital...
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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
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Updated: Apr 13, 2026

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[Multiphoton tomography].

M Zieger1, S Springer, M J Koehler

  • 1Klinik für Hautkrankheiten, Universitätsklinikum Jena, 07743, Jena, Deutschland.

Der Hautarzt; Zeitschrift Fur Dermatologie, Venerologie, Und Verwandte Gebiete
|May 7, 2015
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Summary
This summary is machine-generated.

Multiphoton tomography (MPT) offers noninvasive skin imaging by stimulating natural fluorescence, enabling in vivo diagnostics. This advanced optical biopsy technique provides subcellular resolution for evaluating skin health and diseases.

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Area of Science:

  • Dermatology
  • Biomedical Optics
  • Medical Imaging

Context:

  • Multiphoton tomography (MPT) and microscopy are increasingly vital noninvasive diagnostic tools in dermatology.
  • These techniques utilize biogenic fluorophore stimulation and second harmonic generation for imaging.
  • No exogenous fluorescence staining is required, a key advantage for in vivo human skin examination.

Purpose:

  • To review recent advancements in MPT for in vivo skin evaluation.
  • To highlight MPT's utility in diagnosing dermal diseases and assessing physiological changes.
  • To showcase MPT's role in both clinical diagnostics and biomedical research.

Summary:

  • MPT provides noninvasive optical biopsies up to 200 µm deep with subcellular resolution.
  • It visualizes cellular and extracellular structures without needing additional fluorescence markers.
  • Combining MPT with fluorescence lifetime imaging offers insights into microenvironment, cellular energy, and metabolism.

Impact:

  • MPT enhances in vivo skin diagnostics and biomedical research capabilities.
  • It enables detailed, noninvasive assessment of skin's physiological and pathological conditions.
  • This technology supports the development of new diagnostic strategies for dermal diseases.