Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

15.3K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
15.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fluorescence quenching of nanoemitters within metallic nanoholes.

Optics express·2026
Same author

Clustering-based Failed goal Aware Hindsight Experience Replay.

PeerJ. Computer science·2025
Same author

Under Pressure: Lamina Cribrosa Pore Path Tortuosity in Response to Acute Pressure Modulation.

Translational vision science & technology·2023
Same author

Funnel Devices Based on Asymmetrically Strained Transition Metal Dichalcogenides.

Advanced materials (Deerfield Beach, Fla.)·2023
Same author

Use of nanosecond excitation pulses in fluorescence lifetime measurement via phasor analysis.

Optics express·2022
Same author

In Vivo Sublayer Analysis of Human Retinal Inner Plexiform Layer Obtained by Visible-Light Optical Coherence Tomography.

Investigative ophthalmology & visual science·2022

Related Experiment Video

Updated: Apr 12, 2026

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM
11:57

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

Published on: December 1, 2016

11.3K

Large step-phase measurement by a reduced-phase triple-illumination interferometer.

Behnam Tayebi, Mohammad Reza Jafarfard, Farnaz Sharif

    Optics Express
    |May 14, 2015
    PubMed
    Summary

    A new reduced-phase triple-illumination interferometer (RPTII) enhances optical phase unwrapping precision. This single-shot technique achieves a tenfold decrease in noise for precise phase reconstruction.

    More Related Videos

    Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
    08:39

    Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

    Published on: January 28, 2019

    10.5K
    The Generation of Higher-order Laguerre-Gauss Optical Beams for High-precision Interferometry
    12:14

    The Generation of Higher-order Laguerre-Gauss Optical Beams for High-precision Interferometry

    Published on: August 12, 2013

    22.7K

    Related Experiment Videos

    Last Updated: Apr 12, 2026

    Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM
    11:57

    Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

    Published on: December 1, 2016

    11.3K
    Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
    08:39

    Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

    Published on: January 28, 2019

    10.5K
    The Generation of Higher-order Laguerre-Gauss Optical Beams for High-precision Interferometry
    12:14

    The Generation of Higher-order Laguerre-Gauss Optical Beams for High-precision Interferometry

    Published on: August 12, 2013

    22.7K

    Area of Science:

    • Optical metrology
    • Interferometry
    • Phase measurement

    Background:

    • Dual-illumination techniques improve optical phase unwrapping.
    • Limitations exist in precision and noise reduction for existing methods.

    Purpose of the Study:

    • Introduce a novel single-shot technique, the reduced-phase triple-illumination interferometer (RPTII).
    • Enhance the precision of dual-illumination optical phase unwrapping.
    • Reduce phase reconstruction noise.

    Main Methods:

    • Employing two measurement ranges for low-precision unwrapped and high-precision wrapped phases.
    • Utilizing a hierarchical optical phase unwrapping algorithm.
    • Demonstrating feasibility with a stepped object measurement.

    Main Results:

    • Achieved phase reconstruction without numerical unwrapping algorithms.
    • Reduced noise level by a factor of ten.
    • Successfully measured an object with height 2100 times the wavelength.

    Conclusions:

    • The RPTII is a viable single-shot technique for high-precision phase unwrapping.
    • The method significantly improves phase measurement accuracy and noise reduction.
    • Offers a robust solution for metrology applications requiring precise phase reconstruction.