Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Taipoxin-binding protein on synaptic membranes: identification by affinity labeling.

M C Tzeng1, M J Hseu, C H Yen

  • 1Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan, R.O.C.

Biochemical and Biophysical Research Communications
|December 15, 1989
PubMed
Summary

Researchers identified the neuronal membrane protein responsible for taipoxin binding using affinity labeling. A 45,000 Da protein subunit in neuronal membranes is the primary binding molecule for this neurotoxin.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A real-world experience of a prescribing policy for SGLT2-inhibitors in HFrEF in a Malaysian public tertiary cardiac centre.

The Medical journal of Malaysia·2024
Same author

Application of xenogeneic anti-canine distemper virus antibodies in treatment of canine distemper puppies.

The Journal of small animal practice·2016
Same author

Bacterial conjunctivitis caused by a base eye ointment used as a placebo.

Report (U.S. Naval Medical Research Laboratory)·2014
Same author

Vertical InGaN light-emitting diode with a retained patterned sapphire layer.

Optics express·2013
Same author

Vertical InGaN light-emitting diode with a retained patterned sapphire layer.

Optics express·2012
Same author

Hyaluronic acid instillation following arthroscopic anterior cruciate ligament reconstruction: a double-blinded, randomised controlled study.

Journal of orthopaedic surgery (Hong Kong)·2012

Area of Science:

  • Neuroscience
  • Biochemistry
  • Molecular Biology

Background:

  • Taipoxin is a neurotoxic protein exhibiting phospholipase A2 activity.
  • Understanding taipoxin's neuronal binding is crucial for elucidating its mechanism of action.

Purpose of the Study:

  • To identify the specific neuronal membrane molecules that bind taipoxin.
  • To characterize the binding interaction using affinity labeling techniques.

Main Methods:

  • Affinity labeling of guinea pig brain synaptosomes with radiolabeled [125I]taipoxin.
  • Crosslinking with disuccinimidyl suberate to form covalent conjugates.
  • Analysis of conjugate formation in neuronal versus non-neuronal tissues.

Main Results:

Related Experiment Videos

  • A predominant 60,000 Da radioactive conjugate was formed upon taipoxin binding to synaptosomes.
  • This conjugate was specifically observed in neuronal membranes, not in non-neuronal tissues.
  • A 45,000 Da protein, unique to neuronal membranes, was identified as the major taipoxin binding component.

Conclusions:

  • A specific 45,000 Da protein subunit within neuronal membranes is the primary binding site for taipoxin.
  • This finding advances the understanding of neurotoxin-receptor interactions in the nervous system.