Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Erythropoiesis01:14

Erythropoiesis

6.6K
Red blood cells  (RBCs) transport oxygen to all body tissues. These cells survive only for 120 days and then need to be replenished. Erythropoiesis is the process of RBC production. In healthy individuals, erythropoiesis ensures all tissues are amply supplied with oxygen. In addition, blood loss due to injury leads to a drop in the physiological oxygen level that will cause erythropoiesis. Any defect in erythropoiesis leads to several physiological disorders, including thalassemia, anemia,...
6.6K
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

2.8K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.8K
Factors Affecting Erythropoiesis01:24

Factors Affecting Erythropoiesis

6.6K
The cardiovascular system regulates the number of erythrocytes in the bloodstream to ensure optimal oxygen transport. It also prevents over-proliferation of these cells, which helps to maintain blood viscosity and flow rate.
Several factors influence the erythrocyte production rate, with tissue oxygen level being among the most critical. Intense exercise or high altitudes can cause tissue hypoxia, which triggers the kidneys to release more erythropoietin (EPO) into the bloodstream.
EPO then...
6.6K
Regulation of the Unfolded Protein Response01:31

Regulation of the Unfolded Protein Response

3.2K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
3.2K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

16.8K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.8K
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

2.9K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
2.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Corrigendum to "Integrative multi-scale physiological, metabolic, and proteomic reprogramming in tomato under polyethylene microplastic stress" [Ecotoxicol. Environ. Saf. 316 (2026), 120148].

Ecotoxicology and environmental safety·2026
Same author

simCRISPR: Modeling Experimental Complexity in Pooled CRISPR Screens.

bioRxiv : the preprint server for biology·2026
Same author

CerS2 Is a Druggable Target in Triple-Negative Breast Cancer.

Molecular cancer therapeutics·2026
Same author

Integrative multi-scale physiological, metabolic, and proteomic reprogramming in tomato under polyethylene microplastic stress.

Ecotoxicology and environmental safety·2026
Same author

Effects of oral supplementation with whey protein concentrate and its hydrolysates on blood cholesterol levels and oxidative DNA damage in South Korean male smokers.

Clinical nutrition research·2026
Same author

Influence of prey concentration, light intensity, and temperature on the growth and ingestion of the mixotrophic dinoflagellate Pyrophacus horologium, a predator of the harmful species Heterocapsa niei.

Journal of phycology·2026

Related Experiment Video

Updated: Mar 17, 2026

Direct Lineage Reprogramming of Adult Mouse Fibroblast to Erythroid Progenitors
11:46

Direct Lineage Reprogramming of Adult Mouse Fibroblast to Erythroid Progenitors

Published on: December 14, 2018

6.9K

Iron deficiency upregulates Egr1 expression.

Seung-Min Lee1, Sun Bok Lee, Ron Prywes

  • 1Department of Food and Nutrition, College of Human Ecology, Yonsei University, Seoul, South Korea, leeseungmin@yonsei.ac.kr.

Genes & Nutrition
|May 19, 2015
PubMed
Summary
This summary is machine-generated.

Iron deficiency upregulates Early Growth Response 1 (Egr1) gene expression in hepatoma cells. This occurs via the ERK and Elk-1 signaling pathways, influencing cell death decisions during iron depletion.

More Related Videos

Mouse Fetal Liver Culture System to Dissect Target Gene Functions at the Early and Late Stages of Terminal Erythropoiesis
06:40

Mouse Fetal Liver Culture System to Dissect Target Gene Functions at the Early and Late Stages of Terminal Erythropoiesis

Published on: September 9, 2014

15.9K
Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay
15:32

Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay

Published on: August 5, 2011

34.2K

Related Experiment Videos

Last Updated: Mar 17, 2026

Direct Lineage Reprogramming of Adult Mouse Fibroblast to Erythroid Progenitors
11:46

Direct Lineage Reprogramming of Adult Mouse Fibroblast to Erythroid Progenitors

Published on: December 14, 2018

6.9K
Mouse Fetal Liver Culture System to Dissect Target Gene Functions at the Early and Late Stages of Terminal Erythropoiesis
06:40

Mouse Fetal Liver Culture System to Dissect Target Gene Functions at the Early and Late Stages of Terminal Erythropoiesis

Published on: September 9, 2014

15.9K
Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay
15:32

Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay

Published on: August 5, 2011

34.2K

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Iron-deficient anemia is a common human disease.
  • Understanding iron deficiency's regulatory mechanisms is crucial.

Purpose of the Study:

  • To identify genes regulated by iron deficiency.
  • To elucidate the regulatory mechanisms involved.

Main Methods:

  • cDNA microarrays and qRT-PCR in Hepa1c1c7 cells treated with desferrioxamine (DFO).
  • Reporter assays, cycloheximide treatment, and Western blotting to assess gene activation and signaling pathways.
  • Analysis of reactive oxygen species (ROS) and caspase activity.

Main Results:

  • Iron deficiency (via DFO) significantly upregulated Early Growth Response 1 (Egr1) mRNA levels.
  • Egr1 upregulation involved transcriptional activation through ERK and Elk-1 signaling pathways.
  • DFO-induced iron deficiency reduced ROS and increased cell death via caspase 3/7 activity.

Conclusions:

  • Iron depletion upregulates Egr1 expression in hepatoma cells partly through ERK/Elk-1 signaling.
  • Egr1 regulation by iron deficiency may play a role in cellular fate decisions and cell death.