Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

8.9K
Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
8.9K
Labeling DNA Probes03:31

Labeling DNA Probes

9.8K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
9.8K
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.8K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Surface-Neutralized HgCdSe Quantum Dots for High-Detectivity Infrared Photodetectors.

Nano letters·2026
Same author

A systematic review of methodology in nutritional trials in patients undergoing pancreatoduodenectomy.

Hepatobiliary surgery and nutrition·2026
Same author

Dietary omega-6 lipids promote post-injury aberrant bone formation in obesity.

The Journal of clinical investigation·2026
Same author

Quantum Dot Encoding for In-Solution Single-Molecule Biomarker Counting in Metastatic Prostate Cancer.

ACS nano·2026
Same author

Reporting Standards and Quality Assurance Methods for Pancreatoduodenectomy in Randomised Controlled Trials: A Structured Narrative Review.

Journal of clinical medicine·2026
Same author

Optimisation of electrospinning parameters to successfully obtain high ratios of medium chain length polyhydroxyalkanoate in electrospun fibres with drug loading for wound healing applications.

Journal of materials science. Materials in medicine·2026
Same journal

Mechanism-guided design of photocatalytic MoSâ‚‚ interfaces for antimicrobial applications: From defect engineering to functional surface platforms.

Colloids and surfaces. B, Biointerfaces·2026
Same journal

Glucose-activated enzyme-nanozyme cascade nanoflowers for antibacterial surfaces on cement mortar.

Colloids and surfaces. B, Biointerfaces·2026
Same journal

Ethanol-driven structural transitions of human serum albumin and surface-assisted 2D network formation on Ti-6Al-4V: A kinetic-morphological map.

Colloids and surfaces. B, Biointerfaces·2026
Same journal

Adaptive and intelligent scaffolds: Transforming tissue engineering through material and structural innovations.

Colloids and surfaces. B, Biointerfaces·2026
Same journal

Safeguarding nanovesicles and their payload: A framework for stable storage.

Colloids and surfaces. B, Biointerfaces·2026
Same journal

Drug-loaded nanomicelles with ROS-responsive controlled release of carnosic acid for the treatment of ulcerative colitis.

Colloids and surfaces. B, Biointerfaces·2026
See all related articles

Related Experiment Video

Updated: Apr 12, 2026

Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging
07:38

Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging

Published on: May 17, 2010

23.8K

A self-assembling fluorescent dipeptide conjugate for cell labelling.

Steven Kirkham1, Ian W Hamley1, Andrew M Smith1

  • 1School of Chemistry, Pharmacy and Food Biosciences, University of Reading, Whiteknights, Reading RG6 6AD, UK.

Colloids and Surfaces. B, Biointerfaces
|May 21, 2015
PubMed
Summary
This summary is machine-generated.

A new fluorescein isothiocyanate (FITC) derivative, FITC-LL, is cell-compatible and uptaken by human fibroblasts. This conjugate self-assembles into beta-sheet nanostructures above a critical concentration.

Keywords:
FluorescencePeptide conjugatesPeptidesSelf-assembly

More Related Videos

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time
14:36

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

Published on: August 26, 2009

11.7K
Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
16:16

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Published on: September 13, 2013

15.9K

Related Experiment Videos

Last Updated: Apr 12, 2026

Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging
07:38

Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging

Published on: May 17, 2010

23.8K
Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time
14:36

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

Published on: August 26, 2009

11.7K
Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
16:16

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Published on: September 13, 2013

15.9K

Area of Science:

  • Bioconjugation chemistry
  • Cell biology
  • Nanomaterials science

Background:

  • Fluorescein isothiocyanate (FITC) is a common fluorophore for labeling in bioassays.
  • FITC itself exhibits limited cell uptake and compatibility.
  • Novel FITC derivatives are needed for improved bioimaging and labeling applications.

Purpose of the Study:

  • To synthesize and characterize a novel FITC derivative with enhanced cellular properties.
  • To investigate the cellular uptake, localization, and biocompatibility of the new conjugate.
  • To explore the self-assembly behavior of the FITC derivative at the nanoscale.

Main Methods:

  • Synthesis of an N-terminal leucine dipeptide-FITC conjugate (FITC-LL).
  • Cellular uptake and co-localization studies using human dermal and corneal fibroblasts.
  • Microscopy techniques to observe intracellular localization.
  • Aggregation studies to determine critical aggregation concentration and nanostructure formation.

Main Results:

  • FITC-LL demonstrates significant cell compatibility, unlike unconjugated FITC.
  • Human fibroblasts efficiently uptake FITC-LL.
  • The conjugate localizes to peri-nuclear and intracellular vesicle regions.
  • FITC-LL self-assembles into beta-sheet nanostructures above a critical aggregation concentration.

Conclusions:

  • The FITC-LL conjugate offers improved biocompatibility and cellular uptake compared to FITC.
  • FITC-LL exhibits self-assembly into nanostructures, suggesting potential for novel nanomaterial applications.
  • This derivative shows promise for advanced bioassays and cellular imaging.