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Updated: Apr 12, 2026

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
Published on: October 24, 2016
Xylose fermentation by Saccharomyces cerevisiae using endogenous xylose-assimilating genes
Jin Konishi1, Akira Fukuda, Kozue Mutaguchi
1Bio Fuels & Chemicals R&D Group, Frontier Research Laboratory, Central Technical Research Laboratory, JX Nippon Oil & Energy Corporation, 8, Chidori-cho, Naka-Ku, Yokohama, 231-0815, Japan, jin.konishi@noe.jx-group.co.jp.
Objectives:
To genetically engineer Saccharomyces cerevisiae for improved ethanol productivity from glucose/xylose mixtures.
Results:
An endogenous gene cassette composed of aldose reductase (GRE3), sorbitol dehydrogenase (SOR1) and xylulose kinase (XKS1) with a PGK1 promoter and a terminator was introduced into two S. cerevisiae strains, a laboratory strain (CEN.PK2-1C) and an industrial strain (Kyokai No. 7). The engineered Kyokai No. 7 strain (K7-XYL) exhibited a higher sugar consumption rate (1.03 g l(-1) h(-1)) and ethanol yield (63.8 %) from a glucose and xylose mixture compared to the engineered CEN.PK2-1C strain. Furthermore, K7-XYL produced a larger amount of ethanol (39.6 g l(-1)) compared to K7-SsXYL (32 g l(-1)) with integrated xylose reductase and xylitol dehydrogenase from a xylose-assimilating yeast Scheffersomyces stipitis instead of GRE3 and SOR1.
Conclusion:
The created S. cerevisiae strain showed sufficient xylose-fermenting ability to be used for efficient ethanol production from glucose/xylose.
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