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Peptides from Phage Display Library Modulate Gene Expression in Mesenchymal Cells and Potentiate Osteogenesis in Unicortical Bone Defects

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Dipeptides Increase Functional Activity of Human Skin Fibroblasts.

V V Malinin¹, A O Durnova, V O Polyakova

¹Cytomed Medico-Biological Research-and-Production Complex, St. Petersburg, Russia, Vvm-61@mail.ru.

Bulletin of Experimental Biology and Medicine

|June 3, 2015

Summary

Dipeptides like Glu-Trp stimulate human skin fibroblasts, promoting cell renewal and antioxidant functions. These findings suggest dipeptides are effective for reparative preparations.

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Area of Science:

Biochemistry
Dermatology
Cell Biology

Background:

Human skin fibroblasts are crucial for skin repair and regeneration.
Actovegin is a known preparation used to stimulate cellular processes.
The role of specific dipeptides in modulating fibroblast activity requires further investigation.

Purpose of the Study:

To investigate the effects of dipeptides Glu-Trp and isovaleroyl-Glu-Trp on human skin fibroblast activity.
To compare the efficacy of these dipeptides with Actovegin.
To elucidate the molecular mechanisms underlying dipeptide-induced fibroblast stimulation.

Main Methods:

In vitro analysis of human skin fibroblasts.
Treatment with varying concentrations (0.2, 2, 20 μg/ml) of dipeptides Glu-Trp and isovaleroyl-Glu-Trp.
Assessment of fibroblast functional activity, including cell renewal markers (Ki-67, caspase-9) and antioxidant markers (Hsp-90, inducible NO-synthase).

Main Results:

Dipeptides, particularly Glu-Trp, demonstrated a significant stimulating effect on human skin fibroblasts.
The observed effects of dipeptides were comparable to those of Actovegin.
Dipeptides enhanced cell renewal by increasing Ki-67 synthesis and decreasing caspase-9 expression.
Antioxidant functions were improved through stimulated expression of Hsp-90 and inducible NO-synthase.

Conclusions:

Dipeptides Glu-Trp and isovaleroyl-Glu-Trp show potent stimulating effects on human skin fibroblasts.
These dipeptides activate key pathways involved in cell renewal and antioxidant defense.
Dipeptides represent promising candidates for developing novel preparations aimed at stimulating reparative processes in the skin.