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Detecting the Ligand-binding Domain Dimerization Activity of Estrogen Receptor Alpha Using the Mammalian Two-Hybrid Assay
Published on: December 19, 2018
6.9K
Cell-based assays for screening androgen receptor ligands
Carmela Campana1, Vincenzo Pezzi2, William E Rainey1
1Departments of Molecular and Integrative Physiology and Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan.
Seminars in Reproductive Medicine
|June 4, 2015
Summary
This review compares androgen receptor (AR) cell models for detecting AR bioactivity. Advances in reporter genes and yeast two-hybrid systems have improved AR assay sensitivity and selectivity.
Area of Science:
- Endocrinology
- Molecular Biology
- Pharmacology
Background:
- The androgen receptor (AR) is crucial for mediating androgen effects, regulating target gene transcription upon ligand binding.
- Existing cell-based AR reporter models often face challenges with selectivity due to sequence similarities with other steroid receptors (GR, MR, PGR).
Purpose of the Study:
- To review and compare current cell-based model systems for detecting androgen receptor (AR) bioactivity.
- To highlight advancements improving the sensitivity and selectivity of AR bioassays.
Main Methods:
- Summary and comparison of various cell-based androgen-responsive model systems.
- Discussion of reporter gene technologies and yeast two-hybrid systems for AR detection.
Main Results:
- Significant improvements in AR bioassay sensitivity and selectivity have been achieved.
- Careful selection of cell models, reporter genes, and yeast two-hybrid systems are key factors.
Conclusions:
- Various cell-based models exist for monitoring AR bioactivity.
- Recent advancements offer enhanced sensitivity and selectivity for AR detection compared to older models.
