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High-sensitivity detection of ATP using a localized surface plasmon resonance (LSPR) sensor and split aptamers
Jin-Ho Park1, Ju-Young Byun1, Won-Bo Shim2
1Department of Chemistry, School of Physics and Chemistry, Gwangju Institute of Science and Technology (GIST), 261 Cheomdan-gwagiro, Buk-gu, Gwangju 500-712,Republic of Korea.
Biosensors & Bioelectronics
|June 5, 2015
Summary
A novel localized surface plasmon resonance (LSPR) aptasensor detects adenosine triphosphate (ATP) with high sensitivity. This reusable sensor enables precise ATP quantification in biological samples, including bacterial cells.
Area of Science:
- Nanotechnology and Nanoscience
- Biomedical Engineering
- Analytical Chemistry
Background:
- Accurate detection of adenosine triphosphate (ATP) is crucial for understanding cellular processes.
- Existing methods for ATP detection can be limited in sensitivity, reusability, or applicability to complex biological systems.
Purpose of the Study:
- To develop a highly sensitive and reusable localized surface plasmon resonance (LSPR) aptasensor for ATP detection.
- To demonstrate the sensor's applicability in quantifying ATP in biological samples, such as bacterial cells.
Main Methods:
- Fabrication of a localized surface plasmon resonance (LSPR) aptasensor using gold nanorods (GNRs) functionalized with split ATP aptamers.
- Utilizing the conformational change of aptamers upon ATP binding to induce a measurable LSPR shift.
- Implementing a simple salt-gradient washing protocol for sensor reuse.
Main Results:
- The developed LSPR aptasensor exhibited high sensitivity for ATP detection, with a quantifiable range from 10 pM to 10 μM.
- The sensor demonstrated excellent reusability after a simple washing step, maintaining its performance.
- Successful application in measuring ATP concentrations in E. coli, enabling bacterial cell concentration determination as low as 1.0×10(3) CFU.
Conclusions:
- A novel, highly sensitive, and reusable LSPR aptasensor for ATP detection has been successfully developed.
- The sensor's ability to quantify ATP in bacterial cells highlights its potential for various biological and diagnostic applications.
