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An Ex Vivo Chicken Primary Bursal-cell Culture Model to Study Infectious Bursal Disease Virus Pathogenesis
Published on: October 4, 2018
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Differential Expression Profile of Chicken Embryo Fibroblast DF-1 Cells Infected with Cell-Adapted Infectious Bursal
Raymond K Hui1, Frederick C Leung2
1School of Biological Sciences, The University of Hong Kong, Hong Kong, People's Republic of China.
Plos One
|June 9, 2015
Summary
This study used RNA-Seq to analyze early chicken cell responses to infectious bursal disease virus (IBDV). Key pathways were identified that restrict viral replication and inhibit host cell apoptosis.
Area of Science:
- Virology
- Molecular Biology
- Immunology
Background:
- Infectious Bursal Disease Virus (IBDV) poses a significant threat to poultry health.
- Understanding the early host-pathogen interactions is crucial for developing effective control strategies.
- DF-1 cell line serves as a model for studying avian viral infections.
Purpose of the Study:
- To elucidate the early transcriptional changes in DF-1 cells upon caIBDV infection.
- To identify host-derived molecular mechanisms involved in restricting viral replication.
- To investigate the modulation of host cell processes like apoptosis and endocytosis during early infection.
Main Methods:
- RNA sequencing (RNA-Seq) was employed to profile gene expression.
- Total RNA was extracted from DF-1 cells at 0, 6, and 12 hours post-infection (hpi).
- Bioinformatic analysis was performed on RNA-Seq datasets mapped to the Galgal4.73 reference genome.
Main Results:
- Significant up-regulation of 128 isoforms and down-regulation of 5 isoforms were observed at 12 hpi.
- A novel IFIT5-IRF1/3-RSAD5 pathway was identified, potentially restricting viral replication.
- Modulation of lipid metabolism, membrane fluidity, and inhibition of viral endocytosis were suggested.
- Host apoptotic processes were arrested through the induction of apoptosis inhibitors.
- Genes involved in viral RNA recognition, such as IFIH1, DHX58, and TRIM25, were elevated.
Conclusions:
- RNA-Seq provides a comprehensive view of host-virus molecular interactions during early caIBDV infection.
- Identified pathways and gene modulations highlight host defense mechanisms against IBDV.
- The study reveals early host responses aimed at restricting viral entry and replication.
