Characterisation of anthocyanins in red cabbage using high resolution liquid chromatography coupled with photodiode
Panagiotis Arapitsas1, Per J R Sjöberg2, Charlotta Turner2
1Uppsala University, Department of Physical and Analytical Chemistry, Analytical Chemistry, P.O. Box 599, 751 24 Uppsala, Sweden; University of Florence, Department of Pharmaceutical Sciences, Via Ugo Schiff 6, 50019 Sesto F.no, Florence, Italy.
Abstract:
The aim of this work was to analyse and tentatively identify anthocyanin species in red cabbage using HPLC/DAD-ESI/Qtrap MS. The extraction was realized by using a pressurized liquid technique and the separation of the pigments was achieved by a high resolution liquid chromatography system with a 1.8μm particles C-18 column. Photodiode array detection was employed to determine the UV/Vis spectral characteristic of the pigments. Electrospray ionization-linear ion trap mass spectrometry allowed the specific determination of the fragmentation patterns of the anthocyanins, by performing different ion scan modes. Twenty four anthocyanins were separated and identified, all having cyanidin as aglycon, represented as mono- and/or di-glycoside, and acylated, or not, with aromatic and aliphatic acids. Nine anthocyanins were identified for the first time in red cabbage.
More Related Videos
05:29Profiling Volatile Compounds in Blackcurrant Fruit using Headspace Solid-Phase Microextraction Coupled to Gas Chromatography-Mass Spectrometry
Published on: June 9, 2021
10:22Improved UPLC-UV Method for the Quantification of Vitamin C in Lettuce Varieties Lactuca sativa L. and Crop Wild Relatives Lactuca spp.
Published on: June 30, 2020
