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Updated: Apr 11, 2026
Efficient Mammalian Cell Expression and Single-step Purification of Extracellular Glycoproteins for Crystallization
Published on: December 23, 2015
Expression, purification and crystallization of a family 55 β-1,3-glucanase from Chaetomium thermophilum
Anastassios C Papageorgiou1, Duochuan Li2
1Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, Tykistokatu 6, 20521 Turku, Finland.
Abstract:
A β-1,3-glucanase from the thermophilic fungus Chaetomium thermophilum was overexpressed in Pichia pastoris, purified and crystallized in the presence of 1.8 M sodium/potassium phosphate pH 6.8 as a precipitant. Data to 2.0 Å resolution were collected in-house at 293 K from a single crystal. The crystal was found to belong to space group P2(1), with unit-cell parameters a = 64.1, b = 85.8, c = 68.5 Å, β = 93.1° and one molecule in the asymmetric unit.
