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Related Experiment Video

Updated: Apr 11, 2026

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

Published on: September 28, 2017

Deciphering the protein-RNA recognition code: combining large-scale quantitative methods with structural biology.

Janosch Hennig^1,2, Michael Sattler^1,2

¹Institute of Structural Biology, Helmholtz Zentrum M, ü, nchen, München, Germany.

Bioessays : News and Reviews in Molecular, Cellular and Developmental Biology

|June 11, 2015

Summary

This summary is machine-generated.

RNA binding proteins (RBPs) regulate gene expression through cooperative binding. Combining high-throughput methods with structural biology advances understanding of RNA recognition codes for gene regulation.

Keywords:

CLIP RNA binding proteins RNA recognition motif cooperativity mRNA interactome protein-RNA recognition structural biology

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Last Updated: Apr 11, 2026

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

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Published on: September 28, 2017

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Area of Science:

Molecular Biology
Structural Biology
Genomics

Background:

RNA binding proteins (RBPs) are crucial regulators of gene expression.
Cooperative binding of RBPs to RNA cis-elements is vital for sequence-specific recognition.
A limited number of RBPs can regulate diverse biological activities.

Purpose of the Study:

To review the advancements in understanding protein-RNA recognition.
To highlight the synergy between high-throughput methods and structural biology.

Main Methods:

Cross-linking immuno-precipitation (CLIP) for genome-wide protein-RNA interaction mapping.
High-throughput sequencing methods.
Structural biology techniques for detailed molecular insights.

Main Results:

CLIP and similar methods provide comprehensive maps of protein-RNA interactions.
Structural studies offer insights into RNA recognition mechanisms by RBPs.
Focus has historically been on single RBPs or domains.

Conclusions:

Integrating high-throughput data with structural biology is key to deciphering the protein-RNA recognition code.
This integration will significantly advance the understanding of gene regulation.