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Compound Transfer by Acoustic Droplet Ejection Promotes Quality and Efficiency in Ultra-High-Throughput Screening
Timothy D Dawes1, Rebecca Turincio1, Steven W Jones1
1Department of Biochemical & Cellular Pharmacology, Genentech, South San Francisco, CA, USA.
Journal of Laboratory Automation
|June 17, 2015
Summary
Acoustic droplet ejection (ADE) revolutionizes high-throughput screening by enabling precise, waste-free compound transfers. This technology enhances assay-ready plate generation and dose-response studies, improving data quality and laboratory efficiency.
Area of Science:
- Biochemistry
- Pharmacology
- Laboratory Automation
Background:
- High-throughput screening (HTS) is crucial for drug discovery.
- Traditional compound transfer methods face challenges with precision, waste, and carryover.
- Acoustic droplet ejection (ADE) offers a novel solution for liquid handling.
Purpose of the Study:
- To evaluate the impact and utility of Acoustic droplet ejection (ADE) in high-throughput screening (HTS) workflows.
- To demonstrate ADE's application in generating assay-ready plates and conducting dose-response studies.
- To assess the advantages and disadvantages of ADE in optimizing HTS processes.
Main Methods:
- Utilized two Labcyte Echo ADE liquid handlers within a 1536-well ultra-high-throughput screening (uHTS) system.
- Implemented custom software to manage and leverage data from ADE instrumentation.
- Employed a second automated ADE system to augment uHTS capacity and facilitate secondary assay evaluations.
Main Results:
- ADE facilitated flexible compound formatting, minimized waste, and eliminated compound carryover.
- Successful generation of assay-ready plates for primary screening and dose-response evaluations.
- Demonstrated high-quality data output from ADE across various HTS stages, enhancing overall screening efficiency.
Conclusions:
- Acoustic droplet ejection significantly enhances the efficiency, precision, and flexibility of high-throughput screening operations.
- ADE technology improves data quality and enables effective utilization of compound libraries for drug discovery.
- ADE represents a valuable advancement in laboratory automation for pharmaceutical research and development.
