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Updated: Apr 10, 2026
Author Spotlight: Enhancing Bone Regeneration with Vascularized Artificial Cartilage Integration
Published on: July 14, 2023
Cell engineering by the internalization of bioinstructive micelles for enhanced bone regeneration
Vítor E Santo1,2,3, Juthamas Ratanavaraporn1, Keisuke Sato1
1Department of Biomaterials, Field of Tissue Engineering, Institute for Frontier Medical Sciences, Kyoto University, Sakyo Ku, Kyoto 6068507, Japan.
Aim:
To direct precursor cells toward the osteoblastic lineage, by using an intracellular nanocarrier releasing dexamethasone.
Materials & Methods:
Biodegradable gelatin-based micelles entrapped dexamethasone (dex-micelles). Internalization efficiency and biocompatibility of dex-micelles and their potency for in vitro osteogenic differentiation and in vivo bone regeneration were assessed.
Results:
Dex-micelles were internalized by rat bone marrow mesenchymal stem cells and demonstrated a pH-responsive release profile and an enhancement of 2D and 3D in vitro osteogenic differentiation. In vivo implantation of gelatin scaffolds seeded with rat bone marrow mesenchymal stem cells precultured for 24 h with dex-micelles promoted a significant enhancement of de novo bone formation in a rat ulna defect, in a dose-dependent manner.
Conclusion:
The proposed intracellular delivery system is a powerful tool to promote bone regeneration.
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