Electrochemical droplet-based microfluidics using chip-based carbon paste electrodes for high-throughput analysis in
Akkapol Suea-Ngam1, Poomrat Rattanarat2, Orawon Chailapakul3
1Chromatography and Separation Research Unit (ChSRU), Department of Chemistry, Faculty of Science, Chulalongkorn University, Patumwan, Bangkok 10330, Thailand; Electrochemistry and Optical Spectroscopy Research Unit (EOSRU), Department of Chemistry, Faculty of Science, Chulalongkorn University, Patumwan, Bangkok 10330, Thailand.
Analytica Chimica Acta
|June 20, 2015
Summary
This study introduces a novel microfluidic device for analyzing dopamine (DA) and ascorbic acid (AA) in pharmaceuticals. The method offers high accuracy and reproducibility for drug quality control.
Area of Science:
- Analytical Chemistry
- Microfluidics
- Electrochemistry
Background:
- Accurate quantification of active pharmaceutical ingredients is crucial for drug safety and efficacy.
- Droplet-based microfluidics offers advantages in sample handling and reaction control.
- Electrochemical detection provides sensitive and selective analysis.
Purpose of the Study:
- To develop and validate a droplet-based microfluidic system for the simultaneous determination of dopamine (DA) and ascorbic acid (AA).
- To apply this system for the pharmaceutical analysis of intravenous drugs.
Main Methods:
- Droplet generation in a microfluidic chip using specific oil and aqueous phase flow rates.
- Chronoamperometric detection with chip-based carbon paste electrodes (CPEs) at an optimized potential (150 mV).
- Analysis of DA and AA in pharmaceutical samples.
Main Results:
- High reproducibility with relative standard deviations <5% for intra-day and inter-day measurements.
- Sensitive detection limits: LOD 20 μM (DA), 41 μM (AA); LOQ 70 μM (DA), 137 μM (AA).
- Excellent linearity (R² > 0.998) and accuracy (<±3.0% error) in pharmaceutical samples.
Conclusions:
- The developed droplet-based microfluidic system with CPEs is a robust and accurate method for pharmaceutical analysis.
- This technique enables precise quantification of DA and AA in intravenous drug formulations.
- The study demonstrates a significant advancement in microfluidic applications for drug quality control.
