Related Experiment Video
Updated: Apr 9, 2026
14:53
High-throughput Screening and Biosensing with Fluorescent C. elegans Strains
Published on: May 19, 2011
18.6K
A practical fluorogenic substrate for high-throughput screening of glutathione S-transferase inhibitors
Yuuta Fujikawa1, Fumika Morisaki, Asami Ogura
1School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan. yfuji@toyaku.ac.jp.
Chemical Communications (Cambridge, England)
|June 20, 2015
Abstract:
We report a new fluorogenic substrate for glutathione S-transferase (GST), 3,4-DNADCF, enabling the assay with a low level of nonenzymatic background reaction. Inhibitors against Noppera-bo/GSTe14 from Drosophila melanogaster were identified by high throughput screening using 3,4-DNADCF, demonstrating the utility of this substrate.
