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Related Concept Videos

Photoluminescence: Applications01:14

Photoluminescence: Applications

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Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
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Updated: Apr 9, 2026

A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence FUEL
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Recent progress in luminescent proteins development.

Kenta Saito1, Takeharu Nagai2

  • 1The Center for Brain Integration Research, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8519, Japan.

Current Opinion in Chemical Biology
|June 22, 2015
PubMed
Summary
This summary is machine-generated.

Luminescent proteins offer sensitive and minimally invasive bioimaging, avoiding external light damage common in fluorescence imaging. Recent advances in luminescent proteins, substrates, and indicators enhance brightness and broaden research applications.

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Bioimaging

Background:

  • Bioimaging demands high sensitivity and minimal invasiveness.
  • Fluorescence imaging can cause photo-induced damage or physical reactions due to external light.
  • Luminescence imaging using proteins offers a promising alternative, avoiding external light sources.

Purpose of the Study:

  • To review recent advancements in luminescent protein development for bioimaging.
  • To summarize progress in novel luminescence substrates and indicators.
  • To highlight the improved brightness and applicability of luminescence imaging.

Main Methods:

  • Literature review of recent scientific publications.
  • Analysis of developments in protein engineering for luminescence.
  • Evaluation of new substrate and indicator chemistries.

Main Results:

  • Significant improvements in the brightness of luminescence imaging have been achieved.
  • New luminescent proteins, substrates, and indicators have been developed.
  • These advancements facilitate broader application of luminescence imaging in research.

Conclusions:

  • Luminescence imaging with engineered proteins is a powerful tool for sensitive and non-invasive biological studies.
  • Continued development of luminescent proteins and substrates promises further enhancements in bioimaging capabilities.
  • The field is rapidly advancing, offering new opportunities for researchers.