Simultaneous determination of ascorbic acid, caffeine and paracetamol in drug formulations by differential-pulse...

A simple, rapid and accurate method for the simultaneous determination of ascorbic acid, caffeine and paracetamol in drug formulations has been developed. Peak currents were measured with a glassy carbon electrode at +0.350, +0.618 and +1.425 V versus a saturated calomel electrode for ascorbic acid, paracetamol and caffeine, respectively. Perchloric acid (0.1 M) - methanol (1 + 1) was used both as a solvent and supporting electrolyte. The optimum modulation amplitude, pulse repeat time and scan rate of the polarographic analyser were found to be 50 mV, 0.5 s and 5 mV s-1, respectively and the linear calibration ranges for ascorbic acid, caffeine and paracetamol were 0-35, 0-50, and 0-55 micrograms ml-1, respectively. The relative standard deviations for 9.30 micrograms ml-1 of ascorbic acid, 8.50 micrograms ml-1 of caffeine and 7.30 micrograms ml-1 of paracetamol were 1.3, 2.5 and 0.7%, respectively. Results are reported for several commercially available drugs.