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Updated: Apr 8, 2026

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy
Published on: February 5, 2020
Sortase A-mediated multi-functionalization of protein nanoparticles
Qi Chen1, Qing Sun, Nicholas M Molino
1Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, DE 19716, USA. wilfred@udel.edu.
Abstract:
We report here a new strategy to enable fast, covalent, and site-directed functionalization of protein nanoparticles using Sortase A-mediated ligation using functional proteins ranging from monomeric to large tetrameric structures. Easy purification of the modified E2 nanoparticles is achieved by functionalization with a thermo-responsive elastin-like-peptide. The resulting protein nanoparticles remained intact and active even after repeated phase transitions, suggesting their use in biocatalysis, biosensing, and imaging applications.
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