Related Experiment Video
Updated: Apr 7, 2026
06:40
Mitochondrial Preparation from Microglia for Glycan Analysis
Published on: May 30, 2025
959
Hydrazinonicotinic acid derivatization for selective ionization and improved glycan structure characterization by
Jing Jiao1, Lijun Yang, Ying Zhang
1Shanghai Cancer Center and Key Laboratory of Glycoconjugates Research Ministry of Public Health, Fudan University, Shanghai 200032, P. R. China. luhaojie@fudan.edu.cn ying@fudan.edu.cn.
The Analyst
|July 3, 2015
Summary
This study introduces hydrazinonicotinic acid (HYNIC) derivatization to improve glycan analysis via MALDI-MS, enhancing ionization efficiency and enabling direct detection from glycoproteins for easier structure characterization.
Area of Science:
- Glycomics
- Mass Spectrometry
- Biochemistry
Background:
- Glycan analysis is crucial for understanding cell biology and disease.
- MALDI-MS analysis of glycans is challenging due to low abundance and ionization efficiency.
- Glycan structure characterization is hindered by difficulties in generating informative fragmentations.
Purpose of the Study:
- To develop a novel derivatization method for improved and selective glycan detection using MALDI-MS.
- To enhance the ionization efficiency and signal-to-noise ratio of glycans.
- To enable direct detection and simplified structure elucidation of glycans from complex biological samples.
Main Methods:
- Derivatization of glycans using hydrazinonicotinic acid (HYNIC) at the reducing terminus.
- Analysis of derivatized glycans using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS).
- Utilizing different acid catalysts to obtain complementary fragmentation patterns for structural analysis.
Main Results:
- HYNIC derivatization significantly enhanced glycan ionization efficiency and signal-to-noise ratio (S/N) in both positive and negative modes.
- The method allowed sensitive detection of sialylated glycans.
- HYNIC derivatization enabled direct detection of glycans from glycoproteins without prior separation by suppressing peptide ionization.
- Over 40 N-glycans were successfully identified in human serum samples.
Conclusions:
- HYNIC derivatization is an effective strategy to overcome the limitations of MALDI-MS for glycan analysis.
- This method simplifies glycan detection and facilitates structure elucidation, even in complex biological matrices.
- The approach holds promise for routine glycomic analysis in clinical diagnostics and biological research.
More Related Videos
Related Concept Videos
01:08Matrix-Assisted Laser Desorption Ionization (MALDI)
1.4K
Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...
1.4K
01:19MALDI-TOF Mass Spectrometry
7.7K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
7.7K
01:21Mass Spectrometry: Complex Analysis
2.1K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
2.1K