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Experimental characterization of the transport phenomena, adsorption, and elution in a protein A affinity monolithic

M Omon Herigstad1, Simone Dimartino1, Cristiana Boi1

  • 1Dipartimento di Ingegneria Civile, Chimica, Ambientale e dei Materiali, DICAM, Università di Bologna, via Terracini 28, 40132 Bologna, Italy.

Journal of Chromatography. A
|July 6, 2015
PubMed

A commercially available convective interaction media (CIM) Protein A monolithic column was fully characterized in view of its application for the affinity capture of IgG in monoclonal antibody production processes. By means of moment analysis, the interstitial porosity and axial dispersion coefficient were determined. The frontal analysis method of characteristic points was employed, for the first time with monolithic media, to determine the dynamic binding capacity. The effects of the flow rate and pH on the total recovery of polyclonal IgG and elution profile were evaluated. A comparison with literature data for Protein A chromatography beads demonstrate the superior bed utilization of monolithic media, which gave better performance at lower residence times.

Keywords:
AffinityElutionIgGMonolithsProtein ATransport properties

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