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Updated: Apr 7, 2026

2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes
Published on: August 6, 2018
A simple dual online ultra-high pressure liquid chromatography system (sDO-UHPLC) for high throughput proteome
Hangyeore Lee1, Dong-Gi Mun, Jingi Bae
1Department of Chemistry, Research Institute for Natural Sciences, Korea University, Seoul 136-701, South Korea. sw_lee@korea.ac.kr.
Abstract:
We report a new and simple design of a fully automated dual-online ultra-high pressure liquid chromatography system. The system employs only two nano-volume switching valves (a two-position four port valve and a two-position ten port valve) that direct solvent flows from two binary nano-pumps for parallel operation of two analytical columns and two solid phase extraction (SPE) columns. Despite the simple design, the sDO-UHPLC offers many advantageous features that include high duty cycle, back flushing sample injection for fast and narrow zone sample injection, online desalting, high separation resolution and high intra/inter-column reproducibility. This system was applied to analyze proteome samples not only in high throughput deep proteome profiling experiments but also in high throughput MRM experiments.
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