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Evolution of Orbitrap Mass Spectrometry Instrumentation
Shannon Eliuk1, Alexander Makarov
1Thermo Fisher Scientific, San Jose, California 97232;
Summary
Orbitrap mass spectrometry (MS) has evolved into a leading analytical technique. Its high-resolution, mass accuracy, and sensitivity are crucial for research and routine analysis.
Area of Science:
- Analytical Chemistry
- Mass Spectrometry
- Instrument Development
Background:
- Orbitrap mass spectrometry (MS) emerged in the late 1990s.
- It utilizes electrostatic fields for ion trapping, a novel approach for MS.
Purpose of the Study:
- To trace the historical development of Orbitrap MS.
- To highlight its current prominence and future trends.
- To showcase its application in proteomics and routine analysis.
Main Methods:
- Review of Orbitrap mass analyzer technology.
- Examination of three key instrument families: LTQ Orbitrap, Q Exactive, and Orbitrap Fusion.
- Analysis of technological advancements and applications.
Main Results:
- Orbitrap MS provides high resolution, mass accuracy, and sensitivity.
- Integration with ion injection enhances analytical performance.
- Instruments demonstrate significant advancements over the past decade.
Conclusions:
- Orbitrap MS is a prominent technique in modern analytical science.
- It serves as a versatile (ultra) high-resolution platform.
- Future trends indicate continued innovation and expanded applications in proteomics and targeted analysis.
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