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Fluorescent Probes for H2S Detection and Quantification.

Wei Feng1, Brian W Dymock

  • 1Department of Pharmacy, National University of Singapore, Block S4A #03, 18 Science Drive 4, Singapore, 117543, Singapore.

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Researchers have developed novel fluorescent probes for detecting hydrogen sulfide (H2S). These advanced sensors enable precise quantification of H2S in biological systems, aiding further research into its roles.

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Chemical Biology

Background:

  • Hydrogen sulfide (H2S) is a crucial signaling molecule in biological systems.
  • Accurate detection and quantification of H2S are essential for understanding its physiological roles.
  • Existing detection methods often lack selectivity, especially in complex biological environments with interfering substances.

Purpose of the Study:

  • To review and highlight recent advancements in fluorescent probes for hydrogen sulfide (H2S) detection.
  • To discuss the selectivity and sensitivity of these probes in various biological contexts.
  • To assess the utility of these probes for in vitro, cellular, and in vivo H2S sensing.

Main Methods:

  • Literature review of recently reported fluorescent probes for H2S detection.
  • Analysis of probe selectivity against common thiols and reducing agents.
  • Evaluation of probe sensitivity, with a focus on nanomolar detection limits.
  • Assessment of probe applicability in biological systems, including cell imaging and in vivo studies.

Main Results:

  • Numerous structurally novel and sensitive fluorescent probes for H2S detection have been developed.
  • Many probes demonstrate high selectivity for H2S over other biologically relevant thiols.
  • The most sensitive probes can quantify H2S at concentrations below 100 nM.
  • A growing number of probes are suitable for sensing H2S in living cells, with some exhibiting specific sub-cellular localization.
  • Several probes have been successfully applied in in vivo studies.

Conclusions:

  • A diverse array of sensitive and selective fluorescent probes are now available for H2S detection.
  • These probes facilitate accurate H2S quantification in vitro, in living cells, and in vivo.
  • The availability of these advanced tools empowers biologists to further elucidate the multifaceted roles of H2S in biological processes.