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[Genotype of Rotavirus Vaccine Strain LLR in China is G10P[15]]
Insights
Rotavirus causes severe gastroenteritis in young children, with no specific treatments available. This study identified the Lanzhou lamb rotavirus vaccine (LLR) genotype as G10P[15], clarifying its classification.
Area of Science:
- Virology
- Molecular Biology
- Vaccinology
Context:
- Rotavirus is a primary cause of severe acute gastroenteritis in children globally.
- There is a lack of specific pharmacological treatments for rotavirus infections.
- The Luo Tewei oral vaccine, derived from the Lanzhou lamb rotavirus vaccine (LLR), is the only available rotavirus vaccine in China.
Purpose:
- To accurately determine the genotype of the Lanzhou lamb rotavirus vaccine (LLR) strain.
- To resolve discrepancies in the hypothesized genotype of the LLR vaccine.
Summary:
- This study employed reverse transcription-polymerase chain reaction (RT-PCR), sequencing, alignment, and phylogenetic analyses to genotype the LLR strain.
- The VP7 and VP4 genes of the LLR strain were analyzed.
- The determined genotype of the LLR strain was identified as G10P[15].
Impact:
- Provides definitive genotypic information for the LLR vaccine strain, crucial for vaccine characterization and surveillance.
- Contributes to understanding rotavirus diversity and vaccine strain evolution.
- Informs public health strategies and vaccine development for rotavirus prevention in China and potentially beyond.
Abstract:
Rotavirus is the leading causal agent of severe acute gastroenteritis in children aged <5 years. A specific pharmacologic agent for the treatment of rotavirus-infected children is lacking. In China, only the Luo Tewei oral vaccine (Lanzhou Institute of Biological Products, Shanghai, China), which is produced from Lanzhou lamb rotavirus vaccine (LLR), is available. Studies have hypothesized that the genotype of LLR is G10P[12], To identify the genotype of LLR by reverse transcription-polymerase chain reaction, we showed that the VP7 and VP4 genotypes of LLR were G10 and P[15], respectively, based on sequencing, alignment and phylogenetic analyses. In conclusion, we identified the genotype of rotavirus strain LLR to be G10P[15].
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