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In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay
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A molecular Fe-complex as a catalyst probe for in-gel visual detection of proteins via signal amplification
Sushma Kumari1, Chakadola Panda, Shyamalava Mazumdar
1CReST Chemical Engineering Division, CSIR-National Chemical Laboratory, Pune-411008, India. ss.sengupta@ncl.res.in.
Chemical Communications (Cambridge, England)
|July 16, 2015
Abstract:
We report the use of a molecular peroxidase mimic biuret-Fe-TAML for chemoselective labeling of proteins and the subsequent visual detection (<0.1 pmoles) of the conjugate in a polyacrylamide gel by catalytic signal amplification. Use of this probe in activity based protein profiling (ABPP) of serine proteases is also demonstrated.
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