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Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
Published on: February 9, 2012
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Video-rate two-photon excited fluorescence lifetime imaging system with interleaved digitization
Optics Letters
|July 16, 2015
Summary
A new fluorescence lifetime imaging system achieves video rates for faster biological imaging. This advanced two-photon microscopy technique is compatible with existing microscopes.
Area of Science:
- Biomedical Optics
- Microscopy Technology
- Fluorescence Imaging
Background:
- Two-photon excited fluorescence (TPEF) microscopy enables deep tissue imaging.
- Fluorescence lifetime imaging (FLIM) provides molecular and environmental information.
- Current FLIM systems often lack the speed for dynamic biological processes.
Purpose of the Study:
- To develop a high-speed FLIM system.
- To enable video-rate imaging with TPEF microscopy.
- To demonstrate the system's compatibility with standard microscopes.
Main Methods:
- Implementation of a novel interleaved digitization technique.
- Integration with a standard beam-scanning microscope.
- System validation using laser dyes and biological tissue samples.
Main Results:
- Achieved imaging speeds up to video rate.
- Demonstrated successful proof-of-concept in both phantom and biological samples.
- Confirmed compatibility with existing microscopy hardware.
Conclusions:
- The developed system significantly enhances FLIM speed.
- This advancement facilitates real-time observation of dynamic biological events.
- The system offers a practical upgrade path for TPEF microscopy users.
