Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bacterial Gastroenteritis01:18

Bacterial Gastroenteritis

48
Bacterial gastroenteritis, characterized by diarrhea, abdominal cramps, and vomiting, is often caused by ingestion of contaminated food or water and is frequently associated with pathogenic Escherichia coli strains. These microbes exploit two principal mechanisms to inflict disease.Shiga toxin–producing E. coli, also referred to as STEC—notably O157:H7—release Shiga toxins that target ribosomes, blocking protein synthesis. The B subunit of the toxin binds the host glycolipid...
48
Rous Sarcoma Virus (RSV) and Cancer01:03

Rous Sarcoma Virus (RSV) and Cancer

6.6K
Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...
6.6K
Rous Sarcoma Virus (RSV) and Cancer01:03

Rous Sarcoma Virus (RSV) and Cancer

6.0K
6.0K
Cholera01:25

Cholera

72
Cholera is an acute gastrointestinal disease caused by the Gram-negative bacterium Vibrio cholerae. It is transmitted primarily via the fecal-oral route through the ingestion of contaminated water or food.Vibrio cholerae is a motile, Gram-negative bacterium of the family Vibrionaceae, primarily associated with waterborne outbreaks in areas with inadequate sanitation. Although over 200 serogroups of V. cholerae exist, only O1 and O139 are responsible for epidemic cholera. The O1 serogroup,...
72
Size and Structure of Viral Genomes01:26

Size and Structure of Viral Genomes

1.1K
Viral genomes exhibit remarkable diversity in size, structure, and composition, influencing their replication strategies and interactions with host cells. These genomes consist of either DNA or RNA and may be linear or circular. Additionally, they can be single-stranded or double-stranded, with each configuration affecting how the virus propagates within a host. RNA viruses, for instance, generally have smaller genomes than DNA viruses, a factor that contributes to their high mutation rates and...
1.1K
Viruses with RNA Genomes01:29

Viruses with RNA Genomes

1.4K
RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
1.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rotavirus-specific intestinal resident memory T cells induced by oral vaccination in early-life mice may contribute to protective immunity.

Journal of leukocyte biology·2026
Same author

Human vaccine responses regulated by parallel cytokine pathways.

Nature immunology·2026
Same author

Nur77 Is Associated With Polyfunctional Properties in Virus-Specific Human CD8<sup>+</sup> T Cells.

European journal of immunology·2026
Same author

Virus-like Particles and Spectral Flow Cytometry for Identification of Dengue Virus-Specific B Cells in Mice and Humans.

Viruses·2026
Same author

Prolonged Fever in Pediatric Dengue is Associated With Clinical Severity and Immune Dysregulation.

The Pediatric infectious disease journal·2026
Same author

Development of intestinal tissue-resident T cells in early-life mice before and after weaning.

Scientific reports·2025
Same journal

Structures of the endophytic microbiota during heart rot development in <i>Abies georgei</i> var. <i>smithii</i>.

Microbiology spectrum·2026
Same journal

High <i>Yersinia</i> prevalence in tonsils of wild boars hunted in Northeast Germany using a novel protocol including long cold enrichment.

Microbiology spectrum·2026
Same journal

Genetic variation, recombinant characteristics, and seroprevalence analysis of echovirus 3 causing severe and mild cases of hand, foot, and mouth disease in Guizhou Province.

Microbiology spectrum·2026
Same journal

Mycelial morphology influenced aerobic DNRA in <i>Streptomyces mediolani</i> EM-B2: short rod-shaped mycelium showed markedly greater efficiency than long filamentous mycelium.

Microbiology spectrum·2026
Same journal

Performance and practicality of 16S nanopore sequencing for routine bacterial identification in clinical samples.

Microbiology spectrum·2026
Same journal

Molecular characterization and correlation with β-lactam resistance of penicillin-binding protein2x, 2b, and 1a of <i>Streptococcus pneumoniae</i> in clinical pneumococcal isolates.

Microbiology spectrum·2026
See all related articles

Related Experiment Video

Updated: Apr 6, 2026

Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus
10:49

Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus

Published on: January 28, 2019

9.5K

Rotavirus.

Manuel A Franco1, Harry B Greenberg2

  • 1Facultad de Ciencias y Medicina, Pontificia Universidad Javeriana, Bogotá, Colombia.

Microbiology Spectrum
|July 18, 2015
PubMed
Summary
This summary is machine-generated.

Rotaviruses (RV) cause severe gastroenteritis. Understanding B cell responses to RV is key for developing better vaccines and treatments against this common childhood infection.

More Related Videos

Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins
11:40

Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins

Published on: April 17, 2020

9.6K
Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR
15:16

Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR

Published on: July 22, 2012

17.9K

Related Experiment Videos

Last Updated: Apr 6, 2026

Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus
10:49

Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus

Published on: January 28, 2019

9.5K
Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins
11:40

Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins

Published on: April 17, 2020

9.6K
Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR
15:16

Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR

Published on: July 22, 2012

17.9K

Area of Science:

  • Virology
  • Immunology
  • Public Health

Background:

  • Rotaviruses (RV) are common, infectious viruses causing severe gastroenteritis in children and animals.
  • Antibodies are crucial for infant protection against RV reinfection, but the B cell response is complex.
  • Most circulating B cells with RV-specific antibodies are naive and recognize viral protein VP6 with low affinity.

Purpose of the Study:

  • To elucidate the complex relationship between rotaviruses and B cells (Bc).
  • To understand the characteristics of RV-specific B cells and their antibody gene usage.
  • To explore the implications for developing improved rotavirus vaccines.

Main Methods:

  • Analysis of blood-circulating B cells expressing RV-specific immunoglobulin (Ig).
  • Comparison of RV-specific B cells with non-antigen-specific B cells.
  • Investigation of Ig gene usage in naive and memory B cells.

Main Results:

  • RV-specific B cells are enriched in CD27+ memory B cells (mBc) expressing IgM.
  • Naive and memory RV-specific B cells utilize distinct Ig genes, suggesting different developmental origins.
  • RV B cells can originate from intestinal or systemic immune compartments.

Conclusions:

  • The B cell response to rotavirus involves distinct populations of naive and memory cells.
  • Understanding these B cell dynamics is crucial for improving rotavirus vaccine efficacy.
  • Passive antibody administration shows potential for prophylaxis and therapy.