Related Experiment Video
Updated: Apr 6, 2026

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM
Published on: December 1, 2016
Ensuring long-term stability of infrared camera absolute calibration
Abstract:
Absolute calibration of cryogenic 3-5 µm and 8-10 µm infrared cameras is notoriously instable and thus has to be repeated before actual measurements. Moreover, the signal to noise ratio of the imagery is lowered, decreasing its quality. These performances degradations strongly lessen the suitability of Infrared Imaging. These defaults are often blamed on detectors reaching a different "response state" after each return to cryogenic conditions, while accounting for the detrimental effects of imperfect stray light management. We show here that detectors are not to be blamed and that the culprit can also dwell in proximity electronics. We identify an unexpected source of instability in the initial voltage of the integrating capacity of detectors. Then we show that this parameter can be easily measured and taken into account. This way we demonstrate that a one month old calibration of a 3-5 µm camera has retained its validity.
Related Concept Videos
Instrument Calibration
Analytical Balance Calibration
An analytical balance measures mass and requires regular calibration to...
Glassware Calibration
Volumetric flasks: Volumetric flasks are designed to prepare aqueous solutions of precise volumes accurately with a calibration line on the neck. To calibrate a volumetric flask, it is important to fill it with distilled...
Calibration Curves: Linear Least Squares
For data that follow a straight line, the standard method for fitting is the linear...
Infrared (IR) Spectroscopy: Overview
Different compounds display unique properties due to their...
Uncertainty in Measurement: Reading Instruments
IR Spectrometers

