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Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
Published on: January 5, 2021
Dielectric Spectroscopy of Pressurized Saccharomyces cerevisiae
Szymon Starzonek1, Małgorzata Rutkowska2, Sylwester J Rzoska3
1Institute of Physics & Silesian Intercollegiate Center for Education and Interdisciplinary Research, University of Silesia, ul. 75 Pułku Piechoty 1A, 41-500 Chorzów, Poland.
Abstract:
Results of broadband dielectric spectroscopy (BDS) in Saccharomyces cerevisiae (baker's yeast), in situ as the function of pressure are presented. They show a clear evidence of a threshold to the new pattern of the pressure evolution of the static dielectric permittivity and DC electric conductivity already for P ≈ 200MPa at T = 5 C and P ≈ 300MPa at T = 25 C. BDS monitoring versus pressure tests up to P = 400MPa revealed particularly notable changes of properties after 30 minutes of compressing. Finally, the correlation between the amount of the spectrophotometric maximum absorbance and the DC electric conductivity was found. All these indicate significance of BDS as the tool for testing of pressure properties of cells assemblies, model foods etc., in situ under high pressures.
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