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Interaction of myoglobin with oleic acid
Lifan Shih1, Youngran Chung1, Renuka Sriram1
1Biochemistry and Molecular Medicine, University of California, Davis, Davis CA 95616-8635, United States.
Chemistry and Physics of Lipids
|July 30, 2015
Summary
Oleic acid (OA) binds to myoglobin (Mb), initially specifically and then non-specifically. This suggests myoglobin may transport fatty acids in the body.
Area of Science:
- Biochemistry
- Molecular Biology
Background:
- Palmitate (PA) is known to interact with myoglobin (Mb).
- The interaction of unsaturated fatty acids with myoglobin is less understood.
Purpose of the Study:
- To investigate the interaction between oleic acid (OA), an unsaturated fatty acid, and myoglobin (Mb).
- To determine the nature and extent of OA binding to Mb.
- To explore the potential physiological role of Mb in fatty acid transport.
Main Methods:
- Proton nuclear magnetic resonance ((1)H NMR) spectroscopy was used to monitor Mb signals during OA titration.
- pH titration analysis was performed to understand the binding characteristics.
Main Results:
- (1)H NMR data revealed signal changes in Mb consistent with both specific and non-specific binding of OA.
- At low OA:Mb ratios (<4:1), OA specifically perturbed the 8-heme methyl signal, indicating localized interaction.
- At higher OA:Mb ratios (4:1 to 40:1), OA caused a global perturbation of Mb signals, suggesting non-specific binding.
Conclusions:
- Oleic acid interacts with myoglobin through both specific and non-specific binding mechanisms.
- The observed interactions suggest that myoglobin may function as a fatty acid transporter under specific physiological conditions.
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