Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

2.8K
Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
2.8K
Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

978
Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy  (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used....
978
Atomic Emission Spectroscopy: Instrumentation01:22

Atomic Emission Spectroscopy: Instrumentation

1.6K
The instrumentation of atomic emission spectrometry (AES) involves various components, including atomization devices that convert samples into gas-phase atoms and ions. There are two main types of atomization devices: continuous and discrete atomizers.  Continuous atomizers, like plasmas and flames, introduce samples in a constant stream, while discrete atomizers inject individual samples using syringes or autosamplers. The most common discrete atomizer is the electrothermal atomizer.
1.6K
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

1.7K
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
1.7K
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

2.9K
Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
2.9K
Inductively Coupled Plasma Atomic Emission Spectroscopy: Principle01:19

Inductively Coupled Plasma Atomic Emission Spectroscopy: Principle

2.3K
Inductively coupled plasma (ICP) is the most widely used plasma source in atomic emission spectroscopy (AES), also known as Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES). The ICP source, or torch, consists of three concentric quartz tubes with argon gas flowing through them. A spark from a Tesla coil initiates the ionization of argon, generating a high-temperature plasma.
The ions and electrons produced interact with the fluctuating magnetic field created by a water-cooled...
2.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Curated digital datasets of acid dissociation constants in dipolar aprotic solvents.

RSC advances·2026
Same author

Photoredox-Catalyzed Radical Dideuterofluoromethylation Using a Cyclic Sulfoximine Scaffold.

Organic letters·2026
Same author

Valorization of birch tops and branches containing mechanically inseparable wood and bark.

Green chemistry : an international journal and green chemistry resource : GC·2026
Same author

Acidity Scale in 1,2-Difluorobenzene.

ACS omega·2026
Same author

Probing the Influence of Sulfur-Aromatic Interactions on the Electronic Structure of Gas-Phase Peptides.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same author

In-Depth Analysis of the Data from an Interlaboratory Study of Quantitative Non-Target Screening-How Do the Instrumental Methods Compare?

Molecules (Basel, Switzerland)·2026

Related Experiment Video

Updated: Apr 5, 2026

Characterizing Bacterial Volatiles using Secondary Electrospray Ionization Mass Spectrometry SESI-MS
08:54

Characterizing Bacterial Volatiles using Secondary Electrospray Ionization Mass Spectrometry SESI-MS

Published on: June 8, 2011

18.7K

Transferability of the electrospray ionization efficiency scale between different instruments.

Jaanus Liigand1, Anneli Kruve2, Piia Liigand2

  • 1Institute of Chemistry, Faculty of Science and Technology, University of Tartu, Ravila 14A, 50411, Tartu, Estonia. jaanus.liigand@ut.ee.

Journal of the American Society for Mass Spectrometry
|August 7, 2015
PubMed
Summary
This summary is machine-generated.

Quantitative electrospray ionization efficiencies (IE) are transferable between mass spectrometry instruments. This finding enables consistent IE measurements across different setups, improving analytical reliability.

Keywords:
Different instrumentsESIIonization efficiencyMass spectrometry

More Related Videos

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
09:38

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Published on: November 26, 2013

14.7K
Sample Preparation for Probe Electrospray Ionization Mass Spectrometry
05:47

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry

Published on: February 19, 2020

10.1K

Related Experiment Videos

Last Updated: Apr 5, 2026

Characterizing Bacterial Volatiles using Secondary Electrospray Ionization Mass Spectrometry SESI-MS
08:54

Characterizing Bacterial Volatiles using Secondary Electrospray Ionization Mass Spectrometry SESI-MS

Published on: June 8, 2011

18.7K
Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
09:38

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Published on: November 26, 2013

14.7K
Sample Preparation for Probe Electrospray Ionization Mass Spectrometry
05:47

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry

Published on: February 19, 2020

10.1K

Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry
  • Chemical Analysis

Background:

  • Quantitative electrospray ionization efficiency (IE) is crucial for mass spectrometry.
  • Variability in IE measurements across different instruments hinders data comparability.
  • Standardized IE quantification is needed for reliable analytical results.

Purpose of the Study:

  • To compare quantitative electrospray ionization efficiencies (IE) across diverse mass spectrometry setups.
  • To assess the inter-instrument transferability of IE data.
  • To develop a model for predicting IE values across different instruments.

Main Methods:

  • Quantitative electrospray ionization (ESI) was performed on 15 diverse compounds using four mass analyzers and four ESI sources.
  • Ionization efficiencies (IE), expressed as logIE values, were measured and compared.
  • A linear model using three anchoring points was developed to predict IE values.

Main Results:

  • General trends of IE changes with molecular structure were consistent across all tested setups.
  • IE scales showed minimal statistically significant changes in compound order between instruments (0.21–0.55 log units).
  • Orthogonal ESI source geometry and pneumatic assistance enhanced IE differentiating power.
  • Predicted logIE values using the linear model showed a root mean square error of 0.24–0.72.

Conclusions:

  • Quantitative electrospray ionization efficiency data are transferable between different mass-spectrometric setups.
  • The developed linear model allows for reliable prediction of IE values across instruments.
  • This study establishes a foundation for standardized quantitative IE measurements in mass spectrometry.