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Updated: Apr 5, 2026
11:06
Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE
Published on: October 16, 2017
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Rhamnogalacturonan I modifying enzymes: an update.
Inês R Silva1, Carsten Jers1, Anne S Meyer1
1Center for Bioprocess Engineering, Department of Chemical and Biochemical Engineering, Building 229, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark.
New Biotechnology
|August 10, 2015
Summary
Rhamnogalacturonan I (RGI) modifying enzymes break down plant cell walls. This review details their characteristics, classification, and potential for biorefining applications.
Area of Science:
- Biochemistry
- Enzymology
- Plant Science
Background:
- Rhamnogalacturonan I (RGI) is a key component of plant cell walls.
- RGI-modifying enzymes are crucial for degrading this complex polysaccharide.
Purpose of the Study:
- To consolidate current knowledge on RGI-modifying enzymes.
- To provide an overview of their biocatalytic properties, classification, and structure-function relationships.
Main Methods:
- Review of existing literature on RGI-modifying enzymes.
- Analysis of enzyme properties, including classification (GHF 28, 105; PLF 4, 11) and phylogenetic relationships.
- Discussion of structural features, calcium dependence, and protein engineering for thermostability.
Main Results:
- RGI enzymes include hydrolases (EC 3.2.1.171-174) and lyases (EC 4.2.2.23-24).
- These enzymes are produced by various microbes like Aspergillus spp. and Bacillus subtilis.
- Unique structural features and calcium dependence (especially for lyases) were noted, alongside protein engineering advancements for thermostability.
Conclusions:
- Understanding RGI-modifying enzymes is vital for microbial plant cell wall degradation.
- These enzymes hold significant potential for enzymatic processing and biorefining of plant biomass.
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