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Updated: Apr 5, 2026

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Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
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[INTESTINE MICROBIOTA IN CHILDREN WITH OBESITY AND ALLERGIC DISEASES]
Summary
Changes in children's gut microbiota are linked to obesity and allergies. An increase in Firmicutes and decrease in Bacteroidetes were observed with higher body weight and allergic conditions.
Area of Science:
- Microbiology
- Pediatrics
- Immunology
Background:
- Childhood obesity is a growing global health concern.
- Allergic diseases are prevalent in pediatric populations.
- The gut microbiome plays a crucial role in host health and disease.
Purpose of the Study:
- To investigate the composition of intestinal microflora in children with obesity.
- To evaluate the association between gut microbiota and allergic diseases in children.
Main Methods:
- The study included 66 children aged 3 to 17 years with varying body weights.
- Intestinal microflora analysis was performed following Russian Federation Ministry of Health standards.
- Microbial composition was assessed based on body weight and presence of allergic pathology.
Main Results:
- Healthy children showed increased Firmicutes and decreased Bacteroidetes with higher body weight.
- Allergic children had reduced Bacteroidetes and increased Bacillus and Staphylococcus aureus.
- Clostridium levels increased with rising body mass in all children.
Conclusions:
- Alterations in gut microbiota are associated with the development of obesity in children.
- Changes in intestinal microflora correlate with the presence of allergic conditions in pediatric patients.
- The gut microbiome represents a potential target for managing childhood obesity and allergies.
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