Related Experiment Video

Updated: Apr 5, 2026

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
11:22

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

31.6K

[INTESTINE MICROBIOTA IN CHILDREN WITH OBESITY AND ALLERGIC DISEASES]

O V Maksimoval, E V Zaitseva, S A Mazurina

    Zhurnal Mikrobiologii, Epidemiologii I Immunobiologii
    |August 12, 2015
    PubMed

    Insights

    Changes in children's gut microbiota are linked to obesity and allergies. An increase in Firmicutes and decrease in Bacteroidetes were observed with higher body weight and allergic conditions.

    Area of Science:

    • Microbiology
    • Pediatrics
    • Immunology

    Background:

    • Childhood obesity is a growing global health concern.
    • Allergic diseases are prevalent in pediatric populations.
    • The gut microbiome plays a crucial role in host health and disease.

    Purpose of the Study:

    • To investigate the composition of intestinal microflora in children with obesity.
    • To evaluate the association between gut microbiota and allergic diseases in children.

    Main Methods:

    • The study included 66 children aged 3 to 17 years with varying body weights.
    • Intestinal microflora analysis was performed following Russian Federation Ministry of Health standards.
    • Microbial composition was assessed based on body weight and presence of allergic pathology.

    Main Results:

    • Healthy children showed increased Firmicutes and decreased Bacteroidetes with higher body weight.
    • Allergic children had reduced Bacteroidetes and increased Bacillus and Staphylococcus aureus.
    • Clostridium levels increased with rising body mass in all children.

    Conclusions:

    • Alterations in gut microbiota are associated with the development of obesity in children.
    • Changes in intestinal microflora correlate with the presence of allergic conditions in pediatric patients.
    • The gut microbiome represents a potential target for managing childhood obesity and allergies.
    Abstract

    More Related Videos

    Oral Biofilm Sampling for Microbiome Analysis in Healthy Children10:42

    Oral Biofilm Sampling for Microbiome Analysis in Healthy Children

    Published on: December 31, 2017

    18.1K
    An In Vitro Batch-culture Model to Estimate the Effects of Interventional Regimens on Human Fecal Microbiota07:15

    An In Vitro Batch-culture Model to Estimate the Effects of Interventional Regimens on Human Fecal Microbiota

    Published on: July 31, 2019

    10.6K

    Related Concept Videos

    Development of Human Microbiota01:30

    Development of Human Microbiota

    The human microbiota begins developing at birth and undergoes continual change as we age. Infancy marks a critical period of microbial sensitivity, offering a “window of opportunity” during which beneficial microbes help mature the immune system. By age three, children typically develop a more stable and diverse microbial community. Newborns acquire microbes from their immediate environment; vaginal delivery favors maternal vaginal microbes, while cesarean births favor microbes from...
    37
    Microbiota of the Stomach and Small Intestine01:27

    Microbiota of the Stomach and Small Intestine

    The human gastrointestinal (GI) tract is characterized by distinct physicochemical conditions that shape its microbial communities. Among these, the stomach presents a particularly challenging environment for microbial colonization due to its highly acidic pH, ranging from 1 to 3. This extreme acidity effectively limits microbial density. However, certain acid-tolerant microorganisms are capable of surviving in this niche. Notably, Helicobacter pylori can colonize the gastric mucosa,...
    49
    Microbiota of the Large Intestine01:27

    Microbiota of the Large Intestine

    The large intestine hosts the most densely populated microbial ecosystem in the human body. This complex community primarily consists of anaerobic bacteria, with Bacillota (formerly Firmicutes) and Bacteroidota (formerly Bacteroidetes) as the predominant groups. The distribution of these microbes varies along different sections of the large intestine, influenced by local environmental factors such as oxygen availability and nutrient composition.The cecum, located at the beginning of the large...
    53
    Microbiota of the Respiratory Tract01:29

    Microbiota of the Respiratory Tract

    The human respiratory tract, comprising the upper and lower segments, serves as a critical interface with the external environment. The upper respiratory tract (URT)—including the nostrils, sinuses, pharynx, and oropharynx—is heavily colonized by microbes, while the lower respiratory tract (LRT), composed of the larynx, trachea, bronchi, and lungs, was long thought to be sterile. However, recent molecular studies have revealed that the lungs are not devoid of microbes but act more...
    37
    Introduction to the Human Microbiota01:22

    Introduction to the Human Microbiota

    Microorganisms colonize various regions of the human body, including the mouth, nasal passages, throat, stomach, intestines, urogenital tract, and skin. The total number of microbial cells is estimated to range from 10¹³ to 10¹⁴—comparable to, or exceeding, the number of human somatic cells. This host–microbiome relationship has led to the conceptualization of humans as supraorganisms, wherein microbial communities perform vital roles in development, immunity,...
    79
    Anatomy of the Intestines01:23

    Anatomy of the Intestines

    Although digestion of proteins, carbohydrates, and lipids may begin in the stomach, it is completed in the intestine. The absorption of nutrients, water, and electrolytes from food and drink also occurs in the intestine. The intestines can be divided into two structurally distinct organs—the small and large intestines.
    Small Intestines
    The small intestine is an ~7 meter-long tube with an inner diameter of just 2.5 cm. Since most nutrients are absorbed here, the inner lining of the...
    92.0K