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Updated: Apr 5, 2026
Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis
Published on: May 23, 2021
[PROTEOMIC MASS-SPECTROMETRIC ANALYSIS OF FRESHLY ISOLATED ESCHERICHIA COLI STRAINS]
Aim:
Time-of-flight mass-spectrometric analysis of colon bacilli populations freshly isolated from humans for identification of proteome markers, that are characteristic for hemolytic and non-hemolytic strains, E.coli--associants of opportunistic bacteria.
Materials And Methods:
20 strains of monopopulation of hemolytic E.coli; 100 strains of monopopulation of non-hemolytic E. coli; 15 non-hemolytic strain, isolated from association with opportunistic bacteria. Protein profiling on MALDI-TOF MS Autoflex "Bruker Daltonik" mass-spectrometer.
Results:
Value within species for mass-charge peak with 100% intensity of 9000 Da could be a differential sign for isolation of hemolytic E.coli during mass-spectrometric biotyping.
Conclusion:
Proteomic characteristics of strains from various populations within E.coli are given. Taxon-specific markers for proteomic differentiation of E.coli populations are established.
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