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Updated: Apr 5, 2026
08:36
Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction
Published on: November 1, 2018
32.7K
Dengue Non-coding RNA: TRIMmed for Transmission.
Giel P Göertz1, Gorben P Pijlman1
1Laboratory of Virology, Wageningen University, Droevendaalsesteeg 1, 6708PB, Wageningen, the Netherlands.
Cell Host & Microbe
|August 14, 2015
Summary
Dengue virus produces subgenomic flavivirus RNA (sfRNA) via XRN1 trimming. This sfRNA binds TRIM25, helping the virus evade RIG-I innate immune sensing.
Area of Science:
- Virology
- Immunology
- Molecular Biology
Background:
- Dengue virus infection triggers cellular RNA processing.
- Subgenomic flavivirus RNA (sfRNA) is a key viral product.
- Innate immune sensors like RIG-I detect viral RNA.
Purpose of the Study:
- To investigate the role of sfRNA in Dengue virus pathogenesis.
- To identify host factors interacting with sfRNA.
- To understand mechanisms of viral immune evasion.
Main Methods:
- Analysis of Dengue virus-infected cells.
- RNA sequencing and analysis.
- Protein-RNA interaction assays (e.g., immunoprecipitation).
Main Results:
- XRN1 exoribonuclease trims Dengue virus RNA to generate sfRNA.
- sfRNA accumulates in infected cells.
- sfRNA directly binds to the host protein TRIM25.
Conclusions:
- sfRNA acts as a viral factor to antagonize host immunity.
- Binding of sfRNA to TRIM25 interferes with RIG-I-mediated immune sensing.
- This interaction facilitates Dengue virus immune evasion.
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